亚热带雌性羔羊和母羊卵巢组织的形态特征和卵泡液代谢物

IF 0.4 4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Indian Journal of Animal Research Pub Date : 2024-03-09 DOI:10.18805/ijar.bf-1756
A. Mohammed, S. Al-Suwaiegh, I. Al-Gherair, S. Al-Khamis, S. Al-Awaid, H. Al-Sornokh, W.F. Alhujaili, A. Mohammed
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引用次数: 0

摘要

背景:卵母细胞和卵泡液的回收和评估是辅助生殖技术的关键步骤。本研究旨在评估沙特阿拉伯亚热带地区阿瓦西雌性羔羊和母羊的卵巢组织及其产生的卵母细胞和卵泡液。研究方法收集了 30 只雌性羔羊的生殖系统以及 40 只处于卵泡期和黄体期的母羊的生殖系统。通过卵泡数量、卵母细胞回收率以及卵泡液(FF)代谢物的测定对卵巢组织进行评估。卵泡分为小卵泡(小于 3 毫米)、中卵泡(3-5 毫米)和大卵泡(大于 5 毫米)。抽取中型和大型卵泡采集卵泡液并回收卵母细胞。测定卵泡总蛋白(克/分升)、尿素(毫克/分升)、葡萄糖(毫克/分升)、总胆固醇(毫克/分升)和甘油三酯(毫克/分升)的浓度。结果与雌性羔羊相比,母羊生殖系统和卵巢的重量有所增加。小卵泡的数量在各组之间没有差异,而中卵泡和大卵泡的数量在母羊组中有所增加。与其他组别相比,卵泡期母羊的中等卵泡数量最多。虽然与羔羊相比,母羊的卵母细胞恢复率有所提高,但各组之间的卵母细胞质量并无差异。与雌性羔羊相比,母羊 FF 的总蛋白值较高(P 小于 0.05)。另一方面,与母羔羊的卵母细胞相比,母羔羊卵母细胞的尿素和甘油三酯含量较低(P 小于 0.05)。此外,母羊卵泡期和黄体期的总蛋白、尿素和甘油三酯值相当。此外,与母羔羊卵泡期相比,母羊卵泡期的葡萄糖值略有上升,总胆固醇值略有下降。总之,雌性羔羊和母羊卵巢组织中的卵泡、卵母细胞的恢复和质量以及卵泡液的生化指标都存在差异。回收的卵母细胞和卵泡液可用于辅助生殖技术。
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Morphological Characteristics of Ovarian Tissues and Follicular Fluid Metabolites of Female Lambs and Ewes in Subtropics
Background: Oocyte and follicular fluid recovery and evaluation are a crucial step in assisted reproductive technologies. The aims of the current study were to evaluate the ovarian tissues and their resulting oocytes and follicular fluid of Awassi female lambs and ewes in subtropics of Saudi Arabia. Methods: Thirty reproductive systems of female lambs in addition to forty reproductive systems of ewes of follicular and luteal phases were collected. The ovarian tissues were evaluated through ovarian follicle numbers, oocytes recovery in addition to determination of follicular fluid (FF) metabolites. The ovarian follicles were categorized into small ( less than 3 mm), medium (3-5 mm) and large follicles ( greater than 5 mm). The medium and large ovarian follicles were aspirated for FF collection and oocytes recovery. The FF concentrations of total protein (g/dl), urea (mg/dl), glucose (mg/dl), total cholesterol (mg/dl) and triglycerides (mg/dl) were determined. Result: The weight of reproductive system and ovary were increased of ewes compared to female lambs. The number of small follicles was not differed among groups whereas the numbers of medium and large follicles increase in ewes groups. The highest number of medium follicles was found in ewes of follicular phase compared to other groups. Although the percentage of oocyte recovery increased in ewes compared to lambs, the oocytes quality was not differed among groups. The total protein values were high (P less than 0.05) in FF of ewes compared to that of female lambs. On the other, the values of urea and triglycerides were low (P less than 0.05) in FF of ewes compared to FF of female lambs. Moreover, the values of total protein, urea and triglycerides were comparable in FF of ewes during follicular or luteal phase. Additionally, there is slight increase of glucose and slight decrease of total cholesterol values in FF of ewes compared to FF of female lambs. In conclusion, follicles of ovarian tissues, oocyte recovery and quality and follicular fluid biochemistry of female lambs and ewes showed differences. The recovered oocytes and FF are available for assisted reproductive techniques.
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来源期刊
Indian Journal of Animal Research
Indian Journal of Animal Research AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
1.00
自引率
20.00%
发文量
332
审稿时长
6 months
期刊介绍: The IJAR, the flagship print journal of ARCC, it is a monthly journal published without any break since 1966. The overall aim of the journal is to promote the professional development of its readers, researchers and scientists around the world. Indian Journal of Animal Research is peer-reviewed journal and has gained recognition for its high standard in the academic world. It anatomy, nutrition, production, management, veterinary, fisheries, zoology etc. The objective of the journal is to provide a forum to the scientific community to publish their research findings and also to open new vistas for further research. The journal is being covered under international indexing and abstracting services.
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