探索油杉提取物作为组织病理学应用的天然组织固定剂的潜力

Hassan Chiwar, A. T. Alkali, A. I. Musa, H. B. Umar
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摘要

组织病理学对疾病诊断至关重要,主要依靠有效的组织固定来维持细胞结构。然而,福尔马林的致癌性激发了人们对更安全替代品的兴趣。油辣木因其药用特性而闻名,是一种很有前景的天然固定剂候选物。本研究旨在评估油杉提取物作为组织病理学应用固定剂的可行性,特别关注其与后续组织学分析的兼容性。研究人员用蒸馏水和生理盐水配制了不同浓度的辣木提取物溶液。将安乐死大鼠的肾组织样本与福尔马林一起用这些溶液处理。组织切片经过手工处理、包埋、切片,并使用苏木精和伊红(H&E)技术染色。显微镜检查显示,不同浓度和溶剂的辣木对组织的保存程度不同。在蒸馏水中浓度较高(15%)的 Moringa 表现出最佳的保存效果,类似于福尔马林固定组织,而较低浓度的 Moringa 和生理盐水则表现出次优的固定效果。这些发现凸显了辣木作为组织固定剂的潜力,尤其是在蒸馏水中浓度较高时。建议开展进一步的研究,以分离出活性成分,并探索可替代的辣木提取物来增强组织保存效果。比较研究将阐明辣木提取物固定剂的功效,并为其在组织病理学实践中的应用提供依据。
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EXPLORING THE POTENTIAL OF MORINGA OLEIFERA EXTRACT AS A NATURAL TISSUE FIXATIVE FOR HISTOPATHOLOGICAL APPLICATIONS
Histopathology, crucial for disease diagnosis, heavily relies on effective tissue fixation to maintain cellular architecture. However, the carcinogenic nature of formalin has spurred interest in safer alternatives. Moringa oleifera, renowned for its medicinal properties, presents a promising natural fixative candidate. This study aimed to assess Moringa oleifera extract's viability as a fixative for histopathological applications, specifically focusing on its compatibility with subsequent histological analyses. Distilled water and normal saline solutions of Moringa extract were prepared at varying concentrations. Kidney tissue samples from euthanized rats were treated with these solutions alongside formalin. Tissue sections underwent manual processing, embedding, sectioning, and staining using Hematoxylin and Eosin (H&E) technique. Microscopic examination revealed varying degrees of tissue preservation across Moringa concentrations and solvents. Higher concentrations (15%) in distilled water exhibited optimal preservation, akin to formalin-fixed tissues, while lower concentrations and normal saline solutions showed suboptimal fixation. These findings highlight Moringa oleifera's potential as a tissue fixative, particularly at higher concentrations in distilled water. Further research is recommended to isolate active components and explore alternative Moringa extracts for enhanced tissue preservation. Comparative studies would elucidate the efficacy of Moringa-derived fixatives and inform their utilization in histopathological practice.
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