子宫腺肌症患者异位子宫内膜中糖酵解增强:一项初步研究。

IF 2 4区 医学 Q3 ENDOCRINOLOGY & METABOLISM Gynecological Endocrinology Pub Date : 2024-12-01 Epub Date: 2024-03-27 DOI:10.1080/09513590.2024.2332411
Shiya Huang, Xueqian Huang, Xiaohui Wen, Xuehong Liu, Hongxia Ma, Linling Xie, Yishu Wang, Shanjia Liu, Yongge Guan, Kunyin Li
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引用次数: 0

摘要

研究目的子宫腺肌症以细胞异常增殖、迁移、侵袭、细胞调控和上皮-间质转化等恶性生物学行为为特征,本研究旨在探讨子宫腺肌症的糖酵解活性:2021年1月至2022年8月,本研究共纳入15例因子宫腺肌症接受全子宫切除术的患者和14例患有非子宫内膜疾病的患者,特别是宫颈鳞状上皮内瘤变和子宫肌瘤患者。研究人员收集了子宫腺肌症患者异位内膜的子宫肌层和对照组患者正常的子宫肌层。所有样本均经组织病理学检查确认。样本采用液相色谱-质谱法(LC-MS)、实时定量 PCR、NAD+/NADH 检测试剂盒以及葡萄糖和乳酸检测试剂盒进行分析:结果:腺肌症组患者的子宫肌层中可观察到子宫内膜基质和腺体。我们发现,与对照组的正常子宫肌层相比,子宫腺肌症组异位内膜组织中 HK1、PFKFB3、甘油醛-3-磷酸脱氢酶(GAPDH)、PKM2 和 PDHA 的 mRNA 表达以及 PFKFB3 的蛋白表达均升高。与对照组相比,异位子宫内膜组织中 1,6-二磷酸果糖水平升高,而 NAD + 和 NAD+/NADH 比值下降。此外,还观察到异位子宫内膜的葡萄糖消耗和乳酸生成增加:我们认为,子宫腺肌症妇女异位内膜子宫肌层糖酵解表型的改变可能是子宫腺肌症发病的原因之一。
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Enhanced glycolysis in the myometrium with ectopic endometrium of patients with adenomyosis: a preliminary study.

Objectives: The objective of this study was to investigate the glycolytic activity of adenomyosis, which is characterized by malignant biological behaviors including abnormal cell proliferation, migration, invasion, cell regulation, and epithelial-mesenchymal transition.

Methods: From January 2021 to August 2022, a total of 15 patients who underwent total hysterectomy for adenomyosis and 14 patients who had non-endometrial diseases, specifically with cervical squamous intraepithelial neoplasia and uterine myoma, were included in this study. Myometrium with ectopic endometrium from patients with adenomyosis while normal myometrium from patients in the control group were collected. All samples were confirmed by a histopathological examination. The samples were analyzed by liquid chromatography-mass spectrometry (LC-MS), real-time quantitative PCR, NAD+/NADH assay kit as well as the glucose and lactate assay kits.

Results: Endometrial stroma and glands could be observed within the myometrium of patients in the adenomyosis group. We found that the mRNA expressions of HK1, PFKFB3, glyceraldehyde-3-phospate dehydrogenase (GAPDH), PKM2, and PDHA as well as the protein expressions of PFKFB3 were elevated in ectopic endometrial tissues of the adenomyosis group as compared to normal myometrium of the control group. The level of fructose 1,6-diphosphate was increased while NAD + and NAD+/NADH ratio were decreased compared with the control group. Besides, increased glucose consumption and lactate production were observed in myometrium with ectopic endometrium.

Conclusions: We concluded that altered glycolytic phenotype of the myometrium with ectopic endometrium in women with adenomyosis may contribute the development of adenomyosis.

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来源期刊
Gynecological Endocrinology
Gynecological Endocrinology 医学-妇产科学
CiteScore
4.40
自引率
5.00%
发文量
137
审稿时长
3-6 weeks
期刊介绍: Gynecological Endocrinology , the official journal of the International Society of Gynecological Endocrinology, covers all the experimental, clinical and therapeutic aspects of this ever more important discipline. It includes, amongst others, papers relating to the control and function of the different endocrine glands in females, the effects of reproductive events on the endocrine system, and the consequences of endocrine disorders on reproduction
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