Linc00958 通过调控 microRNA-490-3p 促进肺癌增殖和迁移

IF 2.9 4区 医学 Q1 Medicine Journal of biomedical nanotechnology Pub Date : 2024-04-01 DOI:10.1166/jbn.2024.3817
Kunjie Wang, Lin An, Aimin Zang, Yumiao Li, Yue Huo
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引用次数: 0

摘要

本研究旨在探讨长基因间非蛋白编码RNA 00958(Linc00958)在肺癌(LCa)进展中的作用及其内在机制。研究采用qRT-PCR技术评估了Linc00958在LCa组织和邻近组织中的表达,并通过Kaplan-Meier生存分析法分析了其对患者预后的影响。此外,还在体外检测了 Linc00958 在 LCa 和正常肺细胞系中的表达。为了评估Linc00958基因敲除对LCa细胞的影响,研究人员进行了功能测试,包括CCK-8、EdU和透孔试验。为了揭示其分子机制,研究人员使用双荧光素酶报告实验来证实 Linc00958 与下游基因 microRNA-490-3p 之间的结合关系。为了阐明microRNA-490-3p在Linc00958影响LCa细胞功能中的作用,研究人员进行了共转染实验。结果显示,Linc00958在LCa组织和细胞中过表达,Linc00958的高表达与患者生存率的降低有关。体外实验显示,Linc00958促进了LCa细胞的肿瘤增殖和迁移。计算预测和双荧光素酶报告实验都证明了 microRNA-490-3p 与 Linc00958 之间的结合位点。共转染实验证实,Linc00958 通过调节 microRNA-490-3p 的表达促进了 LCa 细胞的增殖和迁移。综上所述,Linc00958 通过调节 microRNA-490-3p 促进 LCa 细胞的增殖和迁移。
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Linc00958 Promotes Lung Cancer Proliferation and Migration Through Regulating microRNA-490-3p
This study aimed to investigate the role of long intergenic non-protein coding RNA 00958 (Linc00958) in lung cancer (LCa) progression and its underlying mechanism. The study assessed Linc00958 expression in LCa tissues and adjacent tissues using qRT-PCR, and its impact on patient prognosis was analyzed through Kaplan-Meier survival analysis. Additionally, Linc00958 expression in LCa and normal lung cell lines was examined in vitro. Functional assays, including CCK-8, EdU, and transwell assays, were conducted to evaluate the effects of Linc00958 knockdown on LCa cells. To uncover the molecular mechanism, a dual-luciferase reporter assay was used to confirm the binding relationship between Linc00958 and microRNA-490-3p, a downstream gene. Co-transfection experiments were performed to elucidate microRNA-490-3p’s role in Linc00958’s impact on LCa cell functions. The results showed that Linc00958 was overexpressed in LCa tissues and cells, and high Linc00958 expression correlated with reduced patient survival. in vitro experiments revealed that Linc00958 promoted tumor proliferation and migration in LCa cells. Both computational predictions and dual-luciferase reporter assays demonstrated binding sites between microRNA-490-3p and Linc00958. Co-transfection experiments confirmed that Linc00958 facilitated LCa cell proliferation and migration through modulating microRNA-490-3p expression. In summary, Linc00958 promotes LCa cell proliferation and migration by regulating microRNA-490-3p.
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CiteScore
4.30
自引率
17.20%
发文量
145
审稿时长
2.3 months
期刊介绍: Information not localized
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