Adansonia digitata叶和茎提取物的定量植物化学物质和抗氧化潜力

IF 0.2 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Research Journal of Biotechnology Pub Date : 2024-02-29 DOI:10.25303/1904rjbt05013
Chhavi Goel, R. Dutta
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引用次数: 0

摘要

本研究旨在考察猴面包树(Adansonia digitata)茎叶的植物化学特性。对叶和茎提取物的干燥粉末进行了分析,以确定是否存在不同的植物化学物质、黄酮类、萜类、皂苷、单宁、生物碱等。定量分析显示,A. digitata 的叶和茎富含总酚、生物碱、萜类化合物、黄酮类化合物和皂甙。众所周知,这些化合物具有药用潜力和抗氧化活性,这证明猴面包树叶是非洲主要的汤料来源之一。叶绿素(chl.)的估算也通过两种不同的方法对鲜叶和干叶进行了研究。鲜叶的叶绿素含量高达 2.0 毫克/克,而阴干叶和晒干叶的叶绿素含量分别为 1.664 毫克/克和 1.047 毫克/克。使用二甲基亚砜后,鲜叶的叶绿素含量为 2.240 毫克/克,阴干叶和晒干叶的叶绿素含量分别为 1.695 毫克/克和 1.526 毫克/克。这表明植物中的高叶绿素和植物化学物质具有很强的抗氧化性。随后,采用 FRAP 分析法和 DPPH 自由基清除活性法分别测定了 62.5、125、250、500、750 μg/ml 不同浓度提取物的抗氧化活性。乙醇提取物的抗氧化活性高于水乙醇提取物。
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Quantitative Phytochemicals and Antioxidant Potential of Adansonia digitata leaves and stem extract
The objective of this study was to examine the phytochemical properties of the baobab tree (Adansonia digitata) stems and leaves. The dried powder of leaves and stem extract were analyzed for the presence or absence of different phytochemicals, flavonoids, terpenoids, saponins, tannins, alkaloids etc. The quantitative analysis revealed that the leaves and stem of A. digitata are rich in total phenols, alkaloids, terpenoids, flavonoids and saponins. The presence of these compounds was known to show medicinal potential as well as antioxidant activity and this justifies the use of baobab leaves as one of the major sources of soup in Africa. Chlorophyll (chl.) estimation was also studied by two different methods in fresh leaves and dried leaves. Fresh leaves have high chlorophyll content of 2.0 mg/g while shade and sun-dried leaves have content 1.664 mg/g and 1.047 mg/g respectively. With DMSO, high chlorophyll content of fresh leaves is 2.240 mg/g and for shade and sun-dried leaves, it is 1.695 mg/g and 1.526 mg/g respectively. This represents that high chlorophyll and phytochemicals present in plants show high antioxidant properties. Subsequently, antioxidant activity at different concentrations 62.5, 125, 250, 500, 750 μg/ml of the extract was established using FRAP assay and DPPH action radical scavenging activity methods respectively. Ethanolic extract shows higher antioxidant activity than hydroethanolic extract.
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来源期刊
Research Journal of Biotechnology
Research Journal of Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
0.60
自引率
0.00%
发文量
192
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