Farqad M. K. ALdabbagh, I. H. M. AlZaidi, Magida Alshamari
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引用次数: 0
摘要
黑茄科植物黑茄属（Solanum nigrum）的微繁殖和保护采用了离体繁殖方法，以确保其可持续的植物产业和研究可用性。通过在富含不同浓度 BA（苄基腺嘌呤）的 MS 培养基上培养芽尖、节段和叶片，在 1 个月内完成了离体芽分化。与节段外植体和叶片相比，芽尖的芽诱导率和每个外植体的平均芽数有明显差异。在添加了 1 毫克/升 BA 的 MS 培养基上，芽的诱导率最高（100%），每个芽尖的平均微芽数最高（9.200 个）。研究结果表明，在一半的 MS 培养基中添加 I mg/l IBA（吲哚丁酸）时，生根率（100%）令人满意，平均根数（2.600）根/芽，平均根长 8.600 厘米。通过 RAPD 标记对微繁殖植株的遗传稳定性进行了研究，结果表明，与原植株相比，微繁殖植株具有单形性和真型性。
MICROPROPAGATION AND ASSESSMENT OF GENETIC FIDELITY OF REGENERATE BY RAPD MARKERS OF SOLANUM NIGRUM
In vitro propagation approach has been adopted for micropropagation and conservation of Solanum nigrum (black nightshade) to guarantee its sustainable phyto-industry and research availability. Within 1 month, in vitro shoot differentiated was accomplished by culturing shoot tip, nodal segment, and leaf on MS medium enriched with various BA (Benzyl Adenine) concentrations. A significant difference was observed with shoot tips compared with nodal explants and leaf at the shoot induction percentage and mean number of shoot per explant. The highest percentage of shoot induction (100%) and average number of micro shoot per shoo tip (9.200 shoot) were recorded on MS medium fortified with 1 mg/l BA. The findings showed that a satisfactory rooting percentage (100%) was recorded when I mg/l IBA (Indole Butyric Acid) added to half MS medium with an average number of roots (2.600) root/shoot, a mean root length is 8.600cm. rooted plantlets could be successfully acclimatized in the shade house with 100% survival rate. The genetic stability of the micropropagated plants investigated by the RAPD markers indicated monomorphic and true to type when compare with the original plant.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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