使用凝固酶基因法对金黄色葡萄球菌进行基因分型的研究

Q4 Earth and Planetary Sciences Research Journal of Chemistry and Environment Pub Date : 2024-01-31 DOI:10.25303/283rjce040047

Sadah Al-Halfi Alaa S., Al-Shammari Maher Zaki Faisal, Khalil Ibrahim Suaad

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引用次数: 0

摘要

从临床采集的 200 个样本中分离出 50 个金黄色葡萄球菌。金黄色葡萄球菌对苄星青霉素、氧西林和莫西沙星抗生素的耐药性最高，分别为 100%、86%、76%、76%、66%、64%、62%、62%、60% 和 58%，其中对苄星青霉素、氧西林和莫西沙星抗生素的耐药性最高，对左氧氟沙星的耐药性最高，对红霉素的耐药性最高，对四环素的耐药性最高，对妥布霉素的耐药性最高，对庆大霉素的耐药性最高，对克林霉素的耐药性最高，对三甲双胍/磺胺甲噁唑的耐药性最高。利用 PCR 技术检测金黄色葡萄球菌的 Coa 基因时，结果显示存在五种不同大小的 DNA 包。

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Study of Staphylococcus aureus genotyping using Coagulase gene method

Fifty isolates of Staphylococcus aureus bacteria were obtained out of 200 samples collected from clinical sources. The highest resistance to S. aureus bacteria was 100% Benzylpericillin, Oxacillin and Moxiflaxocin antibiotics, 86% Levoflaxacin resistance, 76% Erythromycin resistance, 66% Tetracycline resistance, 64% Tobramycin resistance, 62% Gentamicin resistance, 60% Clindamycin resistance and 58% Trimethoprim/Sulfamethoxazole resistance. It was 38% sensitive to Gentamicin and Clindamycin, 36% sensitive to Tobramycin, 22% sensitive to Erythromycin and 14% sensitive to Levoflaxacin while the results showed the presence of five different sized types of DNA packets when detecting Coa gene of S.aureus bacteria using PCR techniques.

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