以各种加热器为动力的微型检测设备:水媒疾病流行背景下的快速审查

Chuanhao Zhang
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摘要

随着社会的进步和生产力的发展,由水污染引起的水污染和水资源短缺问题日益严重。由病原微生物引起的水媒疾病也给人类和其他生物带来了巨大的危害。这些微生物包括细菌、病毒、原生动物和寄生病原体。传统的检测方法耗时长、成本高,无法满足水资源检测的需要。因此,迫切需要准确、快速、特异、便携的检测设备来检测水中的病原微生物。我们对检测水源性疾病病原微生物的检测方法和检测设备进行了分析和梳理,总结了这些检测方法和检测设备,分析了这些检测方法的优缺点。我们合理地认为,一种好的水中病原微生物检测方法应具有成本低、能耗低、操作简单、特异性强、便携性高等优点,更容易满足水质检测领域的需求。多功能小型核酸检测装置早在几十年前就有报道,它将核酸扩增的反应时间从几小时缩短到几分钟,这些基于核酸扩增的微型装置不仅特异性强,而且成本低廉,非常适合资源有限的环境。
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Miniaturized detection devices powered by various heaters: A quick review under the background of water-borne disease epidemics
With the progress of society and the development of productivity, the problem of water pollution and water shortage caused by water pollution is becoming more and more serious. Water-borne diseases caused by pathogenic microorganisms also bring great harm to human beings and other life forms. These microorganisms include bacteria, viruses, protozoa, and parasitic pathogens. Traditional detection methods are time-consuming and costly, and can’t meet the needs of water resource detection. Therefore, there is an urgent need for accurate, rapid, specific, and portable detection equipment for detecting pathogenic microorganisms in water. We analyzed and sorted out the detection methods and detection equipment for detecting pathogenic microorganisms that cause water-borne diseases, summarized these detection methods and detection equipment, and analyzed the advantages and disadvantages of these detection methods. We reasonably concluded that a good detection method for pathogenic microorganisms in water should have the advantages of low cost, low energy consumption, simple operation, strong specificity, and high portability, which can more easily meet the needs of the water quality detection field. Multifunctional small nucleic acid detection devices have been reported for decades, which reduce the reaction time of nucleic acid amplification from hours to minutes, and these miniaturized devices based on nucleic acid amplification are not only highly specific but also low cost, which is very suitable for resource-limited environments.
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