从土壤样本中分离杀蚊细菌以控制蚊媒

Kakhuangailiu Gangmei, Hemaladkshmi Padmanaban, Aneha Kunnikuruvan, Jibi Lukose, Bhagyashree Bora, Manikandan Sivaprakasam, Abhisubesh Vijayakumar, Sahadiya Mandodan, Mathivanan Ashok kumar, Vijayalakshmi Krishnamoorthy, S. Poopathi
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引用次数: 0

摘要

本研究试图从印度普都切里联邦直辖区采集的不同土壤中筛选和分离出强效的生态友好型灭蚊细菌。在总共 140 份土壤样本中,有 16 个分离物显示出了灭蚊活性。在这 16 个分离菌中,从印度普度克里中央直辖区 Kalapet 和 Kanagachettikulam 村的红色土壤中分离出两种细菌,其代码分别为 PYKAL-31A 和 PYKAL-31A。PYKAL-31A和PYKC-33C最有效。利用这两种细菌菌株对史蒂芬按蚊、埃及伊蚊和库蚊幼虫进行了广泛的生物测定。PYKAL-31A对埃及伊蚊、库蚊和史蒂芬按蚊的半数致死浓度(LC50)和致死浓度(LC90)分别为 0.007、0.011、0.015 和 0.015、0.021 和 0.029;PYKC-33C 的半数致死浓度(LC50)和致死浓度(LC90)分别为 0.009、0.014、0.013 和 0.015、0.026、0.023。
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Isolation of Mosquitocidal Bacteria from Soil Samples to Control Mosquito Vectors
In the present study, an attempt was made to screen and isolate potent and ecofriendly mosquitocidal bacteria from different soils collected from Union Territory of Puducherry, India. From a total of 140 soil samples, 16 isolates showed mosquitocidal activity. Out of these 16 isolates, two bacteria isolated from red soil of Kalapet and Kanagachettikulam villages of Union Territory of Puducherry, India with code no. PYKAL-31A and PYKC-33C were found to be most potent. Extensive bioassay was carried out with these two bacterial strains against larvae of Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus. The LC50 and LC90 values of PYKAL-31A against Aedes aegypti, Culex quinquefasciatus and Anopheles stephensi were 0.007, 0.011, 0.015 and 0.015, 0.021 and 0.029, respectively and that of PYKC-33C were 0.009, 0.014, 0.013 and 0.015, 0.026, 0.023, respectively.
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