加纳制造 用于 DNA 凝胶电泳的琼脂糖

Felix Zoiku, Ameyaw Prince, Agyekum Boateng, Prince Fordjour, Aba Enuson, Malvin Forson, Mina Ansomaa, S. Matrevi, Prince Donkor, Nancy Duah-Quashie, N. Quashie
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引用次数: 0

摘要

这项研究探索了加纳本地海藻生产琼脂糖的潜力,琼脂糖是进口琼脂糖的替代品,可用于 DNA 片段分离的凝胶电泳。使用聚乙二醇、二乙胺基乙基纤维素和二甲亚砜等方法对从 Kpone 和 Labadi 采集的海藻(如 Gracilaria cervicornis 和 Hydropuntia dentata)进行加工,以提取琼脂糖。这些红藻显示出较高的琼脂含量,其中颈丝藻产的琼脂糖较多,凝胶强度和温度特性均优于牙鲆藻。莼菜和杉叶藻中没有琼脂。这项研究证明了本地生产的琼脂糖在脱氧核糖核酸分离中的有效性,表明 "加纳琼脂糖 "的商业化生产具有潜力,可用于分子工作
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Made in Ghana Agarose for gel electrophoresis of DNA
The study explored the potential of local Ghanaian seaweeds in producing agarose, a substitute for imported agarose, for gel electrophoresis in DNA fragment separation. Seaweeds like Gracilaria cervicornis and Hydropuntia dentata , collected from Kpone and Labadi, were processed to extract agarose using methods like polyethylene glycol, diethylaminoethyl cellulose, and dimethyl sulfoxide. These red algae showed high agar content, with Gracilaria cervicornis yielding more agarose with better gel strength and temperature properties than Hydropuntia dentata. No agar was obtained from Ulva fasciata and Caulerpa taxifolia . The study demonstrated the effectiveness of locally produced agarose in deoxyribonucleic acid separation, suggesting potential for commercial production of 'Ghanaian agarose' for molecular work
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