从木槿叶提取物中分离出的新双黄酮类化合物对 MH7A 人类类风湿性关节炎滑膜成纤维细胞具有选择性细胞毒性

IF 2.5 4区医学 Q3 CHEMISTRY, MEDICINAL Journal of Natural Medicines Pub Date : 2024-04-09 DOI:10.1007/s11418-024-01801-6

Kazuki Fujii, Itsuki Iwata, Akiko Takaya, Masami Ishibashi, Yasumasa Hara

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引用次数: 0

摘要

从泰国采集的 Xylia kerrii 中分离出了三种新的双黄酮类化合物（1-3）和两种已知的黄酮类化合物（4、5）。化合物 1-5 对类风湿成纤维细胞样滑膜 MH7A 细胞系具有选择性细胞毒性，这些化合物对人肺部滑膜成纤维细胞 WI-38 VA13 sub 2 RA 细胞系具有微弱的细胞毒性。值得注意的是，化合物 1 对 MH7A 细胞具有高度选择性，其 IC50 值为 6.9 μM，而对 WI-38 VA13 sub 2 RA 细胞的 IC50 值为 100 μM。用化合物 1 处理的 MH7A 细胞的 Western 印迹分析显示 CDKN2A /p16INK4A 和 caspase-8 水平升高。

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New biflavonoids isolated from Xylia kerrii leaves extract with selective cytotoxicity against MH7A human rheumatoid arthritis synovial fibroblast cells

Three new biflavonoids (1–3) and two known flavonoids (4, 5) were isolated from Xylia kerrii collected in Thailand. Compounds 1–5 showed selective cytotoxicity against the rheumatoid fibroblast-like synovial MH7A cell line, and these compounds showed weak cytotoxicity against the human lung synovial fibroblast WI-38 VA13 sub 2 RA cell line. Notably, compound 1 was highly selective toward MH7A cells with an IC₅₀ value of 6.9 μM, whereas the IC₅₀ value for WI-38 VA13 sub 2 RA cells was > 100 μM. The western blotting analysis of MH7A cells treated with compound 1 showed increased CDKN2A /p16INK4A and caspase-8 levels.

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来源期刊

Journal of Natural Medicines 医学-药学

CiteScore

6.90

自引率

3.00%

发文量

审稿时长

1.7 months

期刊介绍： The Journal of Natural Medicines is an international journal publishing original research in naturally occurring medicines and their related foods and cosmetics. It covers: -chemistry of natural products -biochemistry of medicinal plants -pharmacology of natural products and herbs, including Kampo formulas and traditional herbs -botanical anatomy -cultivation of medicinal plants. The journal accepts Original Papers, Notes, Rapid Communications and Natural Resource Letters. Reviews and Mini-Reviews are generally invited.