腺苷酸环化酶级联激活诱导的红细胞膜流动性变化：利用光漂白后荧光恢复进行的研究

IF 2.2 4区生物学 Q3 BIOPHYSICS European Biophysics Journal Pub Date : 2024-04-16 DOI:10.1007/s00249-024-01707-x

A. N. Semenov, A. E. Lugovtsov, S. A. Rodionov, Eu. G. Maksimov, A. V. Priezzhev, E. A. Shirshin

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引用次数: 0

摘要

本研究对亲脂性荧光染料 CM-DiI 标记的红细胞进行了光漂白后荧光恢复（FRAP）实验，以评估腺苷酸环化酶级联激活在红细胞膜脂质横向扩散变化中的作用。用肾上腺素（肾上腺素）或间甲肾上腺素刺激肾上腺素能受体可显著加速 FRAP 恢复，从而表明膜流动性增加。用具有膜渗透性的 cAMP 类似物刺激蛋白激酶 A 也有同样的效果，但不太明显。所观察到的效应假定是由于腺苷酸环化酶信号级联的激活削弱了膜间蛋白和 RBC 细胞骨架之间的相互作用，从而增加了磷脂的流动性。

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Erythrocytes membrane fluidity changes induced by adenylyl cyclase cascade activation: study using fluorescence recovery after photobleaching

In this study, fluorescence recovery after photobleaching (FRAP) experiments were performed on RBC labeled by lipophilic fluorescent dye CM-DiI to evaluate the role of adenylyl cyclase cascade activation in changes of lateral diffusion of erythrocytes membrane lipids. Stimulation of adrenergic receptors with epinephrine (adrenaline) or metaproterenol led to the significant acceleration of the FRAP recovery, thus indicating an elevated membrane fluidity. The effect of the stimulation of protein kinase A with membrane-permeable analog of cAMP followed the same trend but was less significant. The observed effects are assumed to be driven by increased mobility of phospholipids resulting from the weakened interaction between the intermembrane proteins and RBC cytoskeleton due to activation of adenylyl cyclase signaling cascade.

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来源期刊

European Biophysics Journal 生物-生物物理

CiteScore

4.30

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal publishes papers in the field of biophysics, which is defined as the study of biological phenomena by using physical methods and concepts. Original papers, reviews and Biophysics letters are published. The primary goal of this journal is to advance the understanding of biological structure and function by application of the principles of physical science, and by presenting the work in a biophysical context. Papers employing a distinctively biophysical approach at all levels of biological organisation will be considered, as will both experimental and theoretical studies. The criteria for acceptance are scientific content, originality and relevance to biological systems of current interest and importance. Principal areas of interest include: - Structure and dynamics of biological macromolecules - Membrane biophysics and ion channels - Cell biophysics and organisation - Macromolecular assemblies - Biophysical methods and instrumentation - Advanced microscopics - System dynamics.