Langlang Zhang, Xiaofei Wang, Kang Dong, Bin Tan, Xianbo Zheng, Xia Ye, Wei Wang, Jun Cheng, Jiancan Feng
{"title":"串联转录因子 PpNAC1 和 PpNAC5 协同激活 PpPGF 的转录,以调控桃在果实成熟期的软化过程","authors":"Langlang Zhang, Xiaofei Wang, Kang Dong, Bin Tan, Xianbo Zheng, Xia Ye, Wei Wang, Jun Cheng, Jiancan Feng","doi":"10.1007/s11103-024-01429-w","DOIUrl":null,"url":null,"abstract":"<p>Peach fruit rapidly soften after harvest, a significant challenge for producers and marketers as it results in rotting fruit and significantly reduces shelf life. In this study, we identified two tandem genes, <i>PpNAC1</i> and <i>PpNAC5</i>, within the <i>sr</i> (slow ripening) locus. Phylogenetic analysis showed that <i>NAC1</i> and <i>NAC5</i> are highly conserved in dicots and that <i>PpNAC1</i> is the orthologous gene of <i>Non-ripening</i> (<i>NOR</i>) in tomato. <i>PpNAC1</i> and <i>PpNAC5</i> were highly expressed in peach fruit, with their transcript levels up-regulated at the onset of ripening. Yeast two-hybrid and bimolecular fluorescence complementation assays showed PpNAC1 interacting with PpNAC5 and this interaction occurs with the tomato and apple orthologues. Transient gene silencing experiments showed that <i>PpNAC1</i> and <i>PpNAC5</i> positively regulate peach fruit softening. Yeast one-hybrid and dual luciferase assays and LUC bioluminescence imaging proved that PpNAC1 and PpNAC5 directly bind to the <i>PpPGF</i> promoter and activate its transcription. Co-expression of PpNAC1 and PpNAC5 showed higher levels of <i>PpPGF</i> activation than expression of PpNAC1 or PpNAC5 alone. In summary, our findings demonstrate that the tandem transcription factors PpNAC1 and PpNAC5 synergistically activate the transcription of <i>PpPGF</i> to regulate fruit softening during peach fruit ripening.</p>","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":null,"pages":null},"PeriodicalIF":3.9000,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Tandem transcription factors PpNAC1 and PpNAC5 synergistically activate the transcription of the PpPGF to regulate peach softening during fruit ripening\",\"authors\":\"Langlang Zhang, Xiaofei Wang, Kang Dong, Bin Tan, Xianbo Zheng, Xia Ye, Wei Wang, Jun Cheng, Jiancan Feng\",\"doi\":\"10.1007/s11103-024-01429-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Peach fruit rapidly soften after harvest, a significant challenge for producers and marketers as it results in rotting fruit and significantly reduces shelf life. In this study, we identified two tandem genes, <i>PpNAC1</i> and <i>PpNAC5</i>, within the <i>sr</i> (slow ripening) locus. Phylogenetic analysis showed that <i>NAC1</i> and <i>NAC5</i> are highly conserved in dicots and that <i>PpNAC1</i> is the orthologous gene of <i>Non-ripening</i> (<i>NOR</i>) in tomato. <i>PpNAC1</i> and <i>PpNAC5</i> were highly expressed in peach fruit, with their transcript levels up-regulated at the onset of ripening. Yeast two-hybrid and bimolecular fluorescence complementation assays showed PpNAC1 interacting with PpNAC5 and this interaction occurs with the tomato and apple orthologues. Transient gene silencing experiments showed that <i>PpNAC1</i> and <i>PpNAC5</i> positively regulate peach fruit softening. Yeast one-hybrid and dual luciferase assays and LUC bioluminescence imaging proved that PpNAC1 and PpNAC5 directly bind to the <i>PpPGF</i> promoter and activate its transcription. Co-expression of PpNAC1 and PpNAC5 showed higher levels of <i>PpPGF</i> activation than expression of PpNAC1 or PpNAC5 alone. In summary, our findings demonstrate that the tandem transcription factors PpNAC1 and PpNAC5 synergistically activate the transcription of <i>PpPGF</i> to regulate fruit softening during peach fruit ripening.</p>\",\"PeriodicalId\":20064,\"journal\":{\"name\":\"Plant Molecular Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-04-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Molecular Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s11103-024-01429-w\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Molecular Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11103-024-01429-w","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Tandem transcription factors PpNAC1 and PpNAC5 synergistically activate the transcription of the PpPGF to regulate peach softening during fruit ripening
Peach fruit rapidly soften after harvest, a significant challenge for producers and marketers as it results in rotting fruit and significantly reduces shelf life. In this study, we identified two tandem genes, PpNAC1 and PpNAC5, within the sr (slow ripening) locus. Phylogenetic analysis showed that NAC1 and NAC5 are highly conserved in dicots and that PpNAC1 is the orthologous gene of Non-ripening (NOR) in tomato. PpNAC1 and PpNAC5 were highly expressed in peach fruit, with their transcript levels up-regulated at the onset of ripening. Yeast two-hybrid and bimolecular fluorescence complementation assays showed PpNAC1 interacting with PpNAC5 and this interaction occurs with the tomato and apple orthologues. Transient gene silencing experiments showed that PpNAC1 and PpNAC5 positively regulate peach fruit softening. Yeast one-hybrid and dual luciferase assays and LUC bioluminescence imaging proved that PpNAC1 and PpNAC5 directly bind to the PpPGF promoter and activate its transcription. Co-expression of PpNAC1 and PpNAC5 showed higher levels of PpPGF activation than expression of PpNAC1 or PpNAC5 alone. In summary, our findings demonstrate that the tandem transcription factors PpNAC1 and PpNAC5 synergistically activate the transcription of PpPGF to regulate fruit softening during peach fruit ripening.
期刊介绍:
Plant Molecular Biology is an international journal dedicated to rapid publication of original research articles in all areas of plant biology.The Editorial Board welcomes full-length manuscripts that address important biological problems of broad interest, including research in comparative genomics, functional genomics, proteomics, bioinformatics, computational biology, biochemical and regulatory networks, and biotechnology. Because space in the journal is limited, however, preference is given to publication of results that provide significant new insights into biological problems and that advance the understanding of structure, function, mechanisms, or regulation. Authors must ensure that results are of high quality and that manuscripts are written for a broad plant science audience.