艾滋病毒感染免疫学监测方面的先进技术:贝宁艾滋病毒-1 感染者的免疫细胞和细胞因子概况

IF 2.9 4区 医学 Q3 IMMUNOLOGY BMC Immunology Pub Date : 2024-04-20 DOI:10.1186/s12865-024-00615-1
Yaou Pierrot Assogba, Adefounke Prudencia Adechina, Edmond Tchiakpe, Odilon Paterne Nouatin, René K. Kèkè, Moussa Bachabi, Honoré Sourou Bankole, Akadiri Yessoufou
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引用次数: 0

摘要

免疫细胞和细胞因子与艾滋病毒感染期间的病毒血症动态和免疫状态有关。它们可作为监测 HIV-1 感染者(PLHIV-1)的有用生物标志物。本研究旨在评估细胞因子和免疫细胞图谱是否有助于对 PLHIV-1 进行治疗跟踪。在贝宁阿波美-卡拉维/索阿瓦大学医院随访的 40 名治疗成功的 PLHIV-1 (PLHIV-1s) 和 50 名治疗失败的 PLHIV-1 (PLHIV-1f)。另外还招募了 20 名健康人作为对照组。循环细胞因子和免疫细胞分别通过酶联免疫吸附试验和流式细胞术进行量化。与对照组相比,PLHIV-1 组的 CD4 + T 细胞、NK、NKT、粒细胞、经典和非经典单核细胞比例较低,而 CD8 + T 细胞比例较高,尤其是 PLHIV-1f 组。嗜酸性粒细胞、中性粒细胞和 B 细胞的频率在研究组之间没有变化。与 PLHIV-1f 和对照组相比,PLHIV-1s 组的循环 IFN-γ 减少,而 IL-4 则显著增加,尽管 PLHIV-1s 组的艾滋病毒感染降低了对照组的高 Th1 表型。然而,在 PLHIV-1f 中,Th1/Th2 比率仍偏向于 Th1 表型,这表明高病毒载量可能使这些患者保持潜在的促炎状态。炎症细胞因子数据显示,PLHIV-1s 和 PLHIV-1f 组的 IL-6 和 TNF-α 浓度明显高于对照组。与对照组相比,PLHIV-1f 组的 IL-5 和 IL-7 水平明显较高,而 PLHIV-1s 组仅有较高的 IL-5 水平。研究组之间的 IL-13 水平没有变化。我们的研究表明,除了 CD4/CD8 T 细胞比率外,NK 和 NKT 细胞以及 IL-6、TNF-α、IL-5 和 IL-7 细胞因子可作为有价值的免疫生物标志物,用于 PLHIV-1 的治疗监测,尽管需要更多的患者来证实这些结果。
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Advanced in immunological monitoring of HIV infection: profile of immune cells and cytokines in people living with HIV-1 in Benin
Immune cells and cytokines have been linked to viremia dynamic and immune status during HIV infection. They may serve as useful biomarkers in the monitoring of people living with HIV-1 (PLHIV-1). The present work was aimed to assess whether cytokines and immune cell profiles may help in the therapeutic follow-up of PLHIV-1. Forty PLHIV-1 in treatment success (PLHIV-1s) and fifty PLHIV-1 in treatment failure (PLHIV-1f) followed at the University Hospital of Abomey-Calavi/Sô-Ava in Benin were enrolled. Twenty healthy persons were also recruited as control group. Circulating cytokines and immune cells were quantified respectively by ELISA and flow cytometry. PLHIV-1 exhibited low proportions of CD4 + T cells, NK, NKT, granulocytes, classical and non-classical monocytes, and high proportions of CD8 + T cells, particularly in the PLHIV-1f group, compared to control subjects. Eosinophils, neutrophils and B cell frequencies did not change between the study groups. Circulating IFN-γ decreased whereas IL-4 significantly increased in PLHIV-1s compared to PLHIV-1f and control subjects even though the HIV infection in PLHIV-1s downregulated the high Th1 phenotype observed in control subjects. However, Th1/Th2 ratio remained biased to a Th1 phenotype in PLHIV-1f, suggesting that high viral load may have maintained a potential pro-inflammatory status in these patients. Data on inflammatory cytokines showed that IL-6 and TNF-α concentrations were significantly higher in PLHIV-1s and PLHIV-1f groups than in control subjects. Significant high levels of IL-5 and IL-7 were observed in PLHIV-1f compared to controls whereas PLHIV-1s presented only a high level of IL-5. No change was observed in IL-13 levels between the study groups. Our study shows that, in addition to CD4/CD8 T cell ratio, NK and NKT cells along with IL-6, TNF-α, IL-5 and IL-7 cytokines could serve as valuable immunological biomarkers in the therapeutic monitoring of PLHIV-1 although a larger number of patients would be necessary to confirm these results.
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来源期刊
BMC Immunology
BMC Immunology 医学-免疫学
CiteScore
5.50
自引率
0.00%
发文量
54
审稿时长
1 months
期刊介绍: BMC Immunology is an open access journal publishing original peer-reviewed research articles in molecular, cellular, tissue-level, organismal, functional, and developmental aspects of the immune system as well as clinical studies and animal models of human diseases.
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