揭开 RNA 的神秘面纱:m6A 研究技术创新交响曲

IF 2.3 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY Israel Journal of Chemistry Pub Date : 2024-04-26 DOI:10.1002/ijch.202400014
Shengyi Fei, Zheng William Fang, Prof. Boxuan Simen Zhao
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引用次数: 0

摘要

这篇综述介绍了不断发展的 N6-甲基腺苷(m6A)研究方法,强调了先进技术对理解 RNA 表观遗传学的重要性。从 m6A 的基本原理和对高通量方法的需求开始,研究从低通量方法发展到高通量技术,包括抗体依赖和无抗体测序方法,以及基于纳米孔的直接 mRNA 测序和 m6A 检测计算方法。此外,还介绍了 m6A 的空间技术和成像工具。对其特殊应用的讨论强调了绝对定量、单核苷酸分辨率、单分子检测和单细胞剖析的生物学意义。综述最后展望了结合当前技术进行全面 m6A 测序的理想方法,这些方法有望进一步加深我们对基因调控、细胞多样性及其在健康和疾病中的作用的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Unraveling the RNA Tapestry: A Symphony of Innovations in m6A Research Technology

This review navigates the evolving landscape of N6-methyladenosine (m6A) research approaches, emphasizing the importance of advanced technology in understanding RNA epigenetics. Beginning with the fundamentals of m6A and the need for high- throughput methods, the investigation progresses from low-throughput approaches to high-throughput technologies, encompassing antibody-dependent and antibody-free sequencing methods, as well as nanopore-based direct mRNA sequencing and computation methods for m6A detection. Spatial techniques and imaging tools for m6A are also introduced in addition. The discussion of their special applications emphasizes the biological significance of absolute quantification, single-nucleotide resolution, single-molecule detection, and single-cell profiling. The review concludes with a vision of ideal approaches that combine current technologies for comprehensive m6A sequencing, with the potential to further our understanding of gene regulation, cellular diversity, and their roles in health and disease.

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来源期刊
Israel Journal of Chemistry
Israel Journal of Chemistry 化学-化学综合
CiteScore
6.20
自引率
0.00%
发文量
62
审稿时长
6-12 weeks
期刊介绍: The fledgling State of Israel began to publish its scientific activity in 1951 under the general heading of Bulletin of the Research Council of Israel, which quickly split into sections to accommodate various fields in the growing academic community. In 1963, the Bulletin ceased publication and independent journals were born, with Section A becoming the new Israel Journal of Chemistry. The Israel Journal of Chemistry is the official journal of the Israel Chemical Society. Effective from Volume 50 (2010) it is published by Wiley-VCH. The Israel Journal of Chemistry is an international and peer-reviewed publication forum for Special Issues on timely research topics in all fields of chemistry: from biochemistry through organic and inorganic chemistry to polymer, physical and theoretical chemistry, including all interdisciplinary topics. Each topical issue is edited by one or several Guest Editors and primarily contains invited Review articles. Communications and Full Papers may be published occasionally, if they fit with the quality standards of the journal. The publication language is English and the journal is published twelve times a year.
期刊最新文献
Cover Picture: (Isr. J. Chem. 8-9/2024) Special Issue on RNA-Based Catalysts that Revolutionized the Discovery of Bioactive Peptides Hexagonal and Trigonal Quasiperiodic Tilings Breaking the Degeneracy of Sense Codons – How Far Can We Go? Cover Picture: (Isr. J. Chem. 6-7/2024)
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