B. Doukouré, Yann Le Pennec, Cécile Troupin, S. Grayo, M. Eiden, M. Groschup, Noël Tordo, Pierre Roques
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引用次数: 0
摘要
背景:戊型肝炎病毒(HEV)通过排泄物传播,通常是通过人类和/或受感染动物(尤其是猪)体内受污染的水传播。本研究的目的是评估猪血清中抗戊型肝炎病毒抗体的水平,并鉴定猪场猪粪便中的戊型肝炎病毒。研究方法:通过内部 ELISA 和商业 ELISA IDvet 检测 HEV 抗体的存在。通过巢式 RT-PCR 评估 HEV 基因组,然后通过测序(MinION Nanopore 技术)确定基因型。结果:2017-2019年,森林几内亚的血清流行率为43%,明显高于下几内亚地区的7%(P < 0.01)。2022 年在下几内亚地区(科纳克里)进行的二次研究显示存在 HEV 基因型 3c。结论猪体内存在 HEV-3c 要求对人类血清流行率进行评估,并对 HEV 基因型进行人类循环检查。贡献标题:据我们所知,这项研究是几内亚猪HEV感染血清流行率和特征的首次报告。
Seroprevalence and Phylogenetic Characterization of Hepatitis E Virus (Paslahepevirus balayani) in Guinean Pig Population.
Background: Hepatitis E virus (HEV) is transmitted by the fecal route, usually through contaminated water in humans and/or infected animals, especially pigs. The objective of this study was to evaluate the level of anti-HEV antibodies in a panel of pig sera and to identify HEV in pig feces in farms. Methodology: The presence of HEV antibodies was tested by an in-house ELISA and a commercial ELISA IDvet. HEV genome was assessed by nested RT-PCR, and then, genotype was identified by sequencing (MinION Nanopore technology). Results: In 2017-2019, the 43% seroprevalence found in Forest Guinea was significantly higher than the 7% found in the Lower region (p < 0.01). Presence of HEV genotype 3c was demonstrated during a secondary study in the Lower region (Conakry) in 2022. Conclusion: The presence of HEV-3c in pigs calls for an evaluation of seroprevalence in human populations and for a HEV genotype human circulation check. Contribution Heading: This study is the first report, to our knowledge, of seroprevalence and characterization of HEV infection in pigs in Guinea.
期刊介绍:
Vector-Borne and Zoonotic Diseases is an authoritative, peer-reviewed journal providing basic and applied research on diseases transmitted to humans by invertebrate vectors or non-human vertebrates. The Journal examines geographic, seasonal, and other risk factors that influence the transmission, diagnosis, management, and prevention of this group of infectious diseases, and identifies global trends that have the potential to result in major epidemics.
Vector-Borne and Zoonotic Diseases coverage includes:
-Ecology
-Entomology
-Epidemiology
-Infectious diseases
-Microbiology
-Parasitology
-Pathology
-Public health
-Tropical medicine
-Wildlife biology
-Bacterial, rickettsial, viral, and parasitic zoonoses