CONTAMINATION TEST OF ESCHERICHIA COLI AND SALMONELLA TYPHI BACTERIA ON SECANG WOOD EXTRACT GEL (Biancaea sappan)

Objective: Disclosing Agents derived from natural materials are still very minimal. The use of natural materials will increase economic value and have a high level of security. Disclosing solution is the material used to see the presence of plaque. The purpose of the study was to determine the stability of secang wood extract gel preparations based on the biological parameters of Escherichia coli and Salmonella typhi bacteria.   Theoretical framework: Research on secang wood has been widely carried out in research on food and beverage ingredients, fabric coloring and fish tanning, beauty. This research was carried out to obtain natural coloring materials and made them in the form of a disclosing agent (disclosing solution), which is useful for checking the cleanliness of a person's teeth. Disclosing agent (disclosing solution) is a plaque coloring agent used in dentistry with the aim of being able to see the effectiveness of oral hygiene measures or to see the presence of a thin layer on the surface of the teeth (biofilm) known as dental plaque (Dyvta et.al., 2009).   Method: The research is experimental, adult secang wood is taken and washed using sterile aqua pro injection which is flowed clean, drain, dry and make powder. The powder halved is extracted using different solvents namely ethanol and aquades. Continued making gel with an extract concentration of 10% (w/w). HPMC is dissolved into 50 mL of aquadest on a beaker with a temperature of 80-90 oC, stirred slowly to form a gel mass.   Results and conclusion: The results showed that the gel base of the second and third months had contamination, while in the negative control there was contamination from the first to the third month. Statistical tests showed significant differences between the secang wood extract gel group and the positive control and gel bases. Of microbial contamination test results do not contain Escherichia coli contamination and Salmonella typhi bacteria in wood extract gel preparations for the zero month, first month, second month, and third month of storage, this shows that the gel preparation of secang wood extract is stable based on biological parameters.   Implications of the research: The implication of this research is to produce a natural dye that can be used to detect plaque on the surface of teeth.