黑暗剥夺对大鼠肝细胞超微结构和线粒体装置的影响

Morphology Pub Date : 2024-04-09 DOI:10.17816/morph.628955
D. Areshidze
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引用次数: 0

摘要

背景 褪黑素(MT)是一种具有广泛生物活性的激素。褪黑激素固有的多种生物调节作用使其参与了适应性反应的形成和各种疾病的发病机制。据观察,相当一部分人的 MT 分泌会因夜间光照而减少。以前的一些研究表明,褪黑激素缺乏会导致实验动物肝脏结构发生显著变化。但人们对黑暗剥夺条件下肝细胞,特别是线粒体的超微结构特征仍知之甚少。研究目的 研究雄性 Wistar 大鼠在 21 天黑暗剥夺条件下肝脏肝细胞的超微结构特征。材料和方法 研究以 40 只雄性 Wistar 大鼠为对象,分为两组。第 1 组在固定光照条件下饲养。第 2 组在黑暗条件下饲养。使用透射电子显微镜分析肝脏样本。微形态计量法用于评估肝细胞的线粒体装置。使用 GraphPad Prism v8.41 程序(美国)对结果进行统计处理。结果 在 II 组大鼠的肝细胞中,黑暗剥夺导致细胞核形状发生变化,伴随着细胞质肿胀和大量含脂空泡的存在。线粒体的特点是明显增生、大小多态、电子密度高和嵴定向紊乱。在细胞质中,可观察到核糖体从内质网脱落的现象。糖原颗粒的数量明显减少。所研究的线粒体微形态计量参数与对照组相比明显减少。结论 研究表明,黑暗剥夺导致的褪黑激素缺乏会导致肝细胞,尤其是线粒体发生一系列明显的超微结构变化。
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Influence of dark deprivation on the ultrastructure and mitochondrial apparatus of rat hepatocytes
Background Melatonin (MT) is a hormone with a wide range of biological activities. The diversity of biological regulatory effects inherent in MT involves this hormone in the formation of adaptive reactions and in the pathogenesis of various diseases. А decrease оf MT secretion due to exposure to light at night is observed in a significant proportion of people. A number of previous studies have shown that melatonin deficiency, causes significant changes in the structure of the liver of laboratory animals. The state of ultrastructural features of hepatocytes, and in particular their mitochondria, under conditions of dark deprivation remains poorly understood. Aim of study To study the ultrastructural features of liver hepatocytes of male Wistar rats under conditions of 21-day dark deprivation. Materials and methods The study was performed on 40 male Wistar rats, divided into 2 groups. Group 1 was kept under a fixed light regime. Group 2 was kept under dark deprivation conditions. Liver samples, were analyzed using a transmission electron microscope. Micromorphometric methods were used to assess the mitochondrial apparatus of hepatocytes. Statistical processing of the results were performed in the GraphPad Prism v8.41 program (USA). Results In hepatocytes of rats of II group, dark deprivation causes a transformation in the shape of the nuclei, accompanied by swelling of the cytoplasm and the presence of a significant number of lipid-containing vacuoles. Mitochondria are characterized by pronounced hyperplasia, size polymorphism, high electron density, and disordered cristae orientation. In the cytoplasm, the phenomenon of shedding of ribosomes from the endoplasmic reticulum is observed. The number of glycogen granules is significantly reduced. The studied micromorphometric parameters of mitochondria are significantly reduced relative to the control. Conclusion The study suggests that melatonin deficiency, resulting from dark deprivation, leads to a number of significant ultrastructural changes in hepatocytes, especially their mitochondrial apparatus.
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