人类和脊椎动物临时和最终泌尿器官发育过程中的形态发生促进因子

V. А. Аkhmatov, Yu. S. Spirina, D. S. Ledneva, I. А. Aptekar', А. А. Markov, А. N. Steblyuk, V. A. Shidin, G. S. Solovyev, A. R. Nurgalieva, О. G. Solovyeva
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引用次数: 0

摘要

目的是显示脊椎动物(鱼类、鸟类)和人类肾脏稳定状态下形态发生的启动子器官的调控意义。材料和方法对卡内基(SC)12-23 期的 118 个胚胎和 9-12 周胎儿期的 28 个人类胎儿、肉鸡(杂交 "Hibro PG +")孵化 48 小时至 20 天阶段的 268 个胚胎、卵黄囊萎缩阶段(根据 Detlaf,1975 年的分类法,36-37 期)的 50 个鹈鹕鱼苗(Gmelin,1788 年)进行了研究。为了研究光镜观察方法,将材料固定在 10%的中性溶液中,并用石蜡包埋。切片用 Mayer 苏木精和伊红染色,McManus CHIC 法染色。免疫组化法检测 Ki-67、CD31、CD34 阳性细胞。用低真空电子显微镜检查原发性肾脏。在秋明州医疗机构,在对健康女性进行医学流产时,对胚胎和人类胎儿进行了观察,并对紧急工作提出警告。在 "秋明斯基肉鸡 "家禽养殖场孵化车间(秋明州卡斯卡拉村)观察鸡胚胎。对实际材料进行形态分析和统计处理。结果显示研究表明,中间间质的器官分化以及临时和最终泌尿器官肾小球的形成控制着中肾管和中肾憩室的状态。肾间质导管启动肾间质周围中间间质的器官型抗生素,并检测盐性肾间质生成的近端载体。肾小球憩室及其子枝启动中间间质的器官型分化,并控制肾小球生成的扇形机制。肾小球生成的活性 决定免疫细胞汇聚的动态。人类中肾间质腹侧生成的缺失与正常肾脏的生命周期显著缩短、肾间质导管萎缩和免疫细胞活性降低有关。CD31、CD34、Bcl-2、Ki-67 阳性细胞的表达增加与肾小球原基的形成有关,因为肾小球原基在泌尿形成器官的发育过程中会发生移动。结论产前器官形成监测并持续监测形态形成的启动器官--中肾管和中肾滤泡的形成。在鱼类、鸟类和人类的发育过程中,中间间质的器官型感染与免疫细胞汇聚的时序相关。
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Morphogenetic Promoters in the Development of Provisional and Definitive Urinary Organs in Humans and Vertebrates
The aim is to show the regulatory significance of promoter organs of morphogenesis in a stable state of the kidneys of vertebrates (fish, birds) and humans. Material and methods. 118 embryos at stages 12-23 of Carnegie (SC) and 28 human fetuses of 9-12 weeks of fetogenesis, 268 embryos of meat chickens (cross "Hibro PG +") from the stage of 48 hours to 20 days of broiler egg incubation, 50 fry of peled Coregonus peled were studied (Gmelin, 1788) at the stage of yolk sac atrophy (stage 36-37 according to the classification of Detlaf, 1975). To study the method of light microscopy, the material fixed in a 10% neutral form, embedded in paraffin. Sections stained with Mayer's hematoxylin and eosin, McManus CHIC method. Ki-67, CD31, CD34, positive cells are detected by immunohistochemistry. Primary kidney examination by low-vacuum electron microscopy. Embryos and human fetuses are observed in the Tyumen health care facility during medical abortion on demand in anamnestic healthy women with their warning about emergency work. Observation chicken embryos at the poultry farm “Tyumensky Broiler”, incubation workshop (Kaskara village, Tyumen region). Morphometric analysis and statistical processing of the actual material were carried out. Results. It was shown that the organotypic differentiation of the intermediate mesenchyme and the formation of nephrons of the provisional and definitive urinary organs control the state of the mesonephric duct and metanephritic diverticulum. The mesonephric duct initiates the organotypic antibiotic of the perifocal intermediate mesonephric mesenchyme and detects the proximodistal vector of saltatory mesonephronogenesis. The metanephritic diverticulum and its daughter branches initiate the organotypic differentiation of the intermediate mesenchyme and control the fan mechanism of metanephronogenesis. Activity of nephronogenesis Determination of the dynamics of convergence of immunocompetent cells. The absence of ventrodorsal generation of mesonephrons in humans is associated with a significant vital cycle of a normal kidney, atrophy of the mesonephric duct, and a decrease in the activity of immunocompetent cells. An increase in the expression of CD31, CD34, Bcl-2, Ki-67 positive cells is realized in combination with the formation of nephron rudiments as the promoter organs move in the developing organ of urinary formation. Conclusion. Prenatal organogenesis monitors and constantly monitors the formation of promoter organs of morphogenesis - the mesonephric duct and the metanephric disserticulum. Organotypic infection of the intermediate mesenchyme during the development of fish, birds and humans correlates with the chronovector of the convergence of immunocompetent cells.
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