E. Iftitah, Warsito Warsito, Vivi Nurhadianty, Fitri Ariadna Sodi Miranda, Rafika Nur Hafidah
{"title":"使用微波、超声波和酶辅助方法从八角(Illicium verum)中提取反式茴香醚并评估其抗菌活性","authors":"E. Iftitah, Warsito Warsito, Vivi Nurhadianty, Fitri Ariadna Sodi Miranda, Rafika Nur Hafidah","doi":"10.22146/ijc.79341","DOIUrl":null,"url":null,"abstract":"This study examines the efficiency of non-conventional extraction methods to obtain trans-anethole from Illicium verum using single and combination extraction techniques: microwave assisted extraction (MAE), ultrasound-assisted extraction (UAE), and enzyme assisted extraction (EAE) employing lipase from Aspergillus oryzae. All extraction methods were conducted using 96% ethanol (1:5 w/v) with varying time durations. The resulting product was an oleoresin, subsequently analyzed and separated using thin liquid chromatography (TLC) and column chromatography employing a solvent mixture of toluene and ethyl acetate in a 9:1 ratio and identified via gas chromatography-mass spectra (GC-MS). The results revealed that trans-anethole yields from a single extraction method were 30.76% (MAE), 41.05% (UAE), and 40.90% (EAE). The combination of extraction methods, such as MAE-UAE, MAE-EAE, and UAE-EAE, produced trans-anethole yields of approximately 42.73%, 52.80%, and 45.02% respectively, surpassing the yields of the single extraction method. Notably, the triple extraction method of MAE-UAE-EAE yielded the highest trans-anethole content at 56.00%. Antibacterial testing against Staphylococcus aureus was performed on all samples. The trans-anethole demonstrating the highest inhibitory effect was obtained from the double extraction method, particularly the combination of UAE-EAE. These results underscore the synergistic efficiency of combining microwave, ultrasound, and enzymatic extraction methods, highlighting their superior efficacy in obtaining trans-anethole.","PeriodicalId":13515,"journal":{"name":"Indonesian Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Extraction of trans-Anethole from Star Anise (Illicium verum) Using Combination of Microwave, Ultrasonic, and Enzyme Assisted Methods and Evaluation of Their Antibacterial Activity\",\"authors\":\"E. Iftitah, Warsito Warsito, Vivi Nurhadianty, Fitri Ariadna Sodi Miranda, Rafika Nur Hafidah\",\"doi\":\"10.22146/ijc.79341\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study examines the efficiency of non-conventional extraction methods to obtain trans-anethole from Illicium verum using single and combination extraction techniques: microwave assisted extraction (MAE), ultrasound-assisted extraction (UAE), and enzyme assisted extraction (EAE) employing lipase from Aspergillus oryzae. All extraction methods were conducted using 96% ethanol (1:5 w/v) with varying time durations. The resulting product was an oleoresin, subsequently analyzed and separated using thin liquid chromatography (TLC) and column chromatography employing a solvent mixture of toluene and ethyl acetate in a 9:1 ratio and identified via gas chromatography-mass spectra (GC-MS). The results revealed that trans-anethole yields from a single extraction method were 30.76% (MAE), 41.05% (UAE), and 40.90% (EAE). The combination of extraction methods, such as MAE-UAE, MAE-EAE, and UAE-EAE, produced trans-anethole yields of approximately 42.73%, 52.80%, and 45.02% respectively, surpassing the yields of the single extraction method. Notably, the triple extraction method of MAE-UAE-EAE yielded the highest trans-anethole content at 56.00%. Antibacterial testing against Staphylococcus aureus was performed on all samples. The trans-anethole demonstrating the highest inhibitory effect was obtained from the double extraction method, particularly the combination of UAE-EAE. These results underscore the synergistic efficiency of combining microwave, ultrasound, and enzymatic extraction methods, highlighting their superior efficacy in obtaining trans-anethole.\",\"PeriodicalId\":13515,\"journal\":{\"name\":\"Indonesian Journal of Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2024-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indonesian Journal of Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22146/ijc.79341\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indonesian Journal of Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22146/ijc.79341","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Extraction of trans-Anethole from Star Anise (Illicium verum) Using Combination of Microwave, Ultrasonic, and Enzyme Assisted Methods and Evaluation of Their Antibacterial Activity
This study examines the efficiency of non-conventional extraction methods to obtain trans-anethole from Illicium verum using single and combination extraction techniques: microwave assisted extraction (MAE), ultrasound-assisted extraction (UAE), and enzyme assisted extraction (EAE) employing lipase from Aspergillus oryzae. All extraction methods were conducted using 96% ethanol (1:5 w/v) with varying time durations. The resulting product was an oleoresin, subsequently analyzed and separated using thin liquid chromatography (TLC) and column chromatography employing a solvent mixture of toluene and ethyl acetate in a 9:1 ratio and identified via gas chromatography-mass spectra (GC-MS). The results revealed that trans-anethole yields from a single extraction method were 30.76% (MAE), 41.05% (UAE), and 40.90% (EAE). The combination of extraction methods, such as MAE-UAE, MAE-EAE, and UAE-EAE, produced trans-anethole yields of approximately 42.73%, 52.80%, and 45.02% respectively, surpassing the yields of the single extraction method. Notably, the triple extraction method of MAE-UAE-EAE yielded the highest trans-anethole content at 56.00%. Antibacterial testing against Staphylococcus aureus was performed on all samples. The trans-anethole demonstrating the highest inhibitory effect was obtained from the double extraction method, particularly the combination of UAE-EAE. These results underscore the synergistic efficiency of combining microwave, ultrasound, and enzymatic extraction methods, highlighting their superior efficacy in obtaining trans-anethole.
期刊介绍:
Indonesian Journal of Chemistry is a peer-reviewed, open access journal that publishes original research articles, review articles, as well as short communication in all areas of chemistry, including educational chemistry, applied chemistry, and chemical engineering.