活木微生物群采样方法

IF 6.3 2区 环境科学与生态学 Q1 ECOLOGY Methods in Ecology and Evolution Pub Date : 2024-04-22 DOI:10.1111/2041-210X.14311
Wyatt Arnold, Jonathan Gewirtzman, Peter A. Raymond, Mark A. Bradford, Claire Butler, Jordan Peccia
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引用次数: 0

摘要

描述不同环境中微生物生命特征的工作取得了巨大进展,但地球上最大的生物质库--活树木的木材--的微生物群组在很大程度上尚未得到探索。目前对树木微生物群的了解主要局限于根和叶,很少关注木材的内生微生物群,尽管新出现的研究表明这一区域是独特类群的生存环境,对生态系统健康和全球生物地球化学循环具有重要影响。缺乏调查的部分原因是木材的物理不稳定性,这给取样、均质化和提取核酸带来了挑战。针对这些问题,我们提出了一种用于微生物分析的木材处理优化方法,从取样到下游分析。我们使用甲烷循环类群作为模式内生菌,评估了我们的方法在回收过程中的损失,并确定了每 100 毫克(干)木材中约 500 个细胞的检测极限。对于所有六个被评估的物种--代表了硬木和软木的多个不同类群--PCR 抑制作用被证明是最小的,我们预计这种方法适用于大多数树种。本文介绍的方法有助于今后对木材微生物组和甲烷循环的全球微生物生态学进行研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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A method for sampling the living wood microbiome

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来源期刊
CiteScore
11.60
自引率
3.00%
发文量
236
审稿时长
4-8 weeks
期刊介绍: A British Ecological Society journal, Methods in Ecology and Evolution (MEE) promotes the development of new methods in ecology and evolution, and facilitates their dissemination and uptake by the research community. MEE brings together papers from previously disparate sub-disciplines to provide a single forum for tracking methodological developments in all areas. MEE publishes methodological papers in any area of ecology and evolution, including: -Phylogenetic analysis -Statistical methods -Conservation & management -Theoretical methods -Practical methods, including lab and field -This list is not exhaustive, and we welcome enquiries about possible submissions. Methods are defined in the widest terms and may be analytical, practical or conceptual. A primary aim of the journal is to maximise the uptake of techniques by the community. We recognise that a major stumbling block in the uptake and application of new methods is the accessibility of methods. For example, users may need computer code, example applications or demonstrations of methods.
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