智利蓝贻贝(Mytilus chilensis)中两个类似蒸汽机的转座子的鉴定和表达分析

IF 4.3 2区 生物学 Q1 BIOLOGY Biological Research Pub Date : 2024-04-26 DOI:10.1186/s40659-024-00498-x
Gloria Arriagada, Johan Quezada, Nicolas Merino-Veliz, Fernando Avilés, Diana Tapia-Cammas, Jorge Gomez, Daniela Curotto, Juan A. Valdes, Pablo A. Oyarzún, Cristian Gallardo-Escárate, Michael J. Metzger, Marco Alvarez
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引用次数: 0

摘要

播散性肿瘤病(DN)是双壳类软体动物循环系统的一种细胞增生性疾病。这种疾病可在个体间传播,也可由溴脱氧尿苷等外部化学物质诱发。我们克隆并鉴定了一种名为 Steamer 的 LTR 反转座子。Steamer mRNA水平和基因拷贝数与DN相关,可用作该疾病的标记。迄今为止,逆转录转座子的表达与 DN 有关的软体动物只有 Mya arenaria。另一方面,有报道称智利蓝贻贝(Mytilus chilensis)也会感染 DN。我们的目的是鉴定智利贻贝中的逆转录转座子,并研究它们在播散性肿瘤中的表达水平。在此,我们表明,2018 年 8 月从两个养殖区采集的个体中有 7.1%呈现出 DN 所描述的形态特征。利用Steamer序列询问M. chilensis的转录组,我们发现了两个推定的逆转录转座子,命名为Steamer样元件(MchSLEs)。MchSLEs 存在于 M. chilensis 的基因组中,而且 MchSLE1 确实是一个 LTR 逆转录转座子。所报道的 MchSLEs 的表达和拷贝数均与 DN 状态无关,但两者在不同动物个体中的表达水平不同。我们还报告说,在培养的 M. chilensis 血细胞中,溴脱氧尿苷可诱导 MchSLEs1 的表达。我们的结论是,存在于智利贻贝中的 SLEs 在不同个体中的表达量不同,而且与播散性肿瘤无关。用溴脱氧尿苷等应激源处理血细胞可诱导 MchSLE1 的表达,这表明在智利贻贝中,环境应激源可诱导 LTR 反转座子的活化。
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Identification and expression analysis of two steamer-like retrotransposons in the Chilean blue mussel (Mytilus chilensis)
Disseminated neoplasia (DN) is a proliferative cell disorder of the circulatory system of bivalve mollusks. The disease is transmitted between individuals and can also be induced by external chemical agents such as bromodeoxyuridine. In Mya arenaria, we have cloned and characterized an LTR-retrotransposon named Steamer. Steamer mRNA levels and gene copy number correlates with DN and can be used as a marker of the disease. So far, the only mollusk where a retrotransposon expression relates to DN is Mya arenaria. On the other hand, it has been reported that the Chilean blue mussel Mytilus chilensis can also suffers DN. Our aim was to identify retrotransposons in Mytilus chilensis and to study their expression levels in the context of disseminated neoplasia. Here we show that 7.1% of individuals collected in August 2018, from two farming areas, presents morphological characteristics described in DN. Using Steamer sequence to interrogate the transcriptome of M. chilensis we found two putative retrotransposons, named Steamer-like elements (MchSLEs). MchSLEs are present in the genome of M. chilensis and MchSLE1 is indeed an LTR-retrotransposon. Neither expression, nor copy number of the reported MchSLEs correlate with DN status but both are expressed at different levels among individual animals. We also report that in cultured M. chilensis haemocytes MchSLEs1 expression can be induced by bromodeoxyuridine. We conclude that SLEs present in Mytilus chilensis are differentially expressed among individuals and do not correlate with disseminated neoplasia. Treatment of haemocytes with a stressor like bromodeoxyuridine induces expression of MchSLE1 suggesting that in Mytilus chilensis environmental stressors can induce activation of LTR-retrotransposon.
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来源期刊
Biological Research
Biological Research 生物-生物学
CiteScore
10.10
自引率
0.00%
发文量
33
审稿时长
>12 weeks
期刊介绍: Biological Research is an open access, peer-reviewed journal that encompasses diverse fields of experimental biology, such as biochemistry, bioinformatics, biotechnology, cell biology, cancer, chemical biology, developmental biology, evolutionary biology, genetics, genomics, immunology, marine biology, microbiology, molecular biology, neuroscience, plant biology, physiology, stem cell research, structural biology and systems biology.
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