{"title":"甘蔗渣提取物中葡萄糖和木糖的高效液相色谱法的开发与验证","authors":"Saeedeh Jafari, Abdollah Hematian Sourki","doi":"10.1093/chromsci/bmae018","DOIUrl":null,"url":null,"abstract":"A validated rapid high-performance liquid chromatography (HPLC)-refractive index (RI) method was developed for the identification and quantification of glucose and xylose in hydrolyzed bagasse extract. The separation of compounds was achieved on Eurokat® H column (300 × 8 mm, 10 μm) at 75°C, using 0.01 N sulfuric acid solution as mobile phase and 0.6 mL/min as flow rate. The method was validated based on accuracy, precision, linearity, robustness, uncertainty, limit of detection (LOD) and limit of quantification (LOQ). Total chromatographic analysis time per sample was ~11 min. Calibration plots were linear over the concentration ranges 11–100 μg/100 μL for glucose and xylose. The LOD was 0.8 ppm and LOQ was 2.5 ppm. The high recovery and low relative standard deviation confirm the suitability of the method for determination of glucose and xylose in bagasse extract. The proposed HPLC-RI method was accurate, fast and robust and required less run time due to less analytes retention times and allowed optimal energy consumption owing to lower column oven temperature.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development and Validation of the HPLC Method for Simultaneous Quantification of Glucose and Xylose in Sugar Cane Bagasse Extract\",\"authors\":\"Saeedeh Jafari, Abdollah Hematian Sourki\",\"doi\":\"10.1093/chromsci/bmae018\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A validated rapid high-performance liquid chromatography (HPLC)-refractive index (RI) method was developed for the identification and quantification of glucose and xylose in hydrolyzed bagasse extract. The separation of compounds was achieved on Eurokat® H column (300 × 8 mm, 10 μm) at 75°C, using 0.01 N sulfuric acid solution as mobile phase and 0.6 mL/min as flow rate. The method was validated based on accuracy, precision, linearity, robustness, uncertainty, limit of detection (LOD) and limit of quantification (LOQ). Total chromatographic analysis time per sample was ~11 min. Calibration plots were linear over the concentration ranges 11–100 μg/100 μL for glucose and xylose. The LOD was 0.8 ppm and LOQ was 2.5 ppm. The high recovery and low relative standard deviation confirm the suitability of the method for determination of glucose and xylose in bagasse extract. The proposed HPLC-RI method was accurate, fast and robust and required less run time due to less analytes retention times and allowed optimal energy consumption owing to lower column oven temperature.\",\"PeriodicalId\":15430,\"journal\":{\"name\":\"Journal of chromatographic science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2024-05-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of chromatographic science\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1093/chromsci/bmae018\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of chromatographic science","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1093/chromsci/bmae018","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
摘要
为鉴定和定量水解甘蔗渣提取物中的葡萄糖和木糖,开发了一种有效的快速高效液相色谱-折光率(RI)分析方法。采用 Eurokat® H 色谱柱(300 × 8 mm, 10 μm),以 0.01 N 硫酸溶液为流动相,流速为 0.6 mL/min,检测温度为 75°C。该方法的准确度、精密度、线性、稳健性、不确定性、检出限(LOD)和定量限(LOQ)均得到了验证。每个样品的色谱分析总时间约为 11 分钟。葡萄糖和木糖在 11-100 μg/100 μL 的浓度范围内线性关系良好。LOD 为 0.8 ppm,LOQ 为 2.5 ppm。高回收率和低相对标准偏差证实了该方法适用于蔗渣提取物中葡萄糖和木糖的测定。所提出的 HPLC-RI 方法准确、快速、稳健,由于分析物的保留时间较短,所需的运行时间也较短,而且由于柱温较低,能耗也较低。
Development and Validation of the HPLC Method for Simultaneous Quantification of Glucose and Xylose in Sugar Cane Bagasse Extract
A validated rapid high-performance liquid chromatography (HPLC)-refractive index (RI) method was developed for the identification and quantification of glucose and xylose in hydrolyzed bagasse extract. The separation of compounds was achieved on Eurokat® H column (300 × 8 mm, 10 μm) at 75°C, using 0.01 N sulfuric acid solution as mobile phase and 0.6 mL/min as flow rate. The method was validated based on accuracy, precision, linearity, robustness, uncertainty, limit of detection (LOD) and limit of quantification (LOQ). Total chromatographic analysis time per sample was ~11 min. Calibration plots were linear over the concentration ranges 11–100 μg/100 μL for glucose and xylose. The LOD was 0.8 ppm and LOQ was 2.5 ppm. The high recovery and low relative standard deviation confirm the suitability of the method for determination of glucose and xylose in bagasse extract. The proposed HPLC-RI method was accurate, fast and robust and required less run time due to less analytes retention times and allowed optimal energy consumption owing to lower column oven temperature.
期刊介绍:
The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.