伊朗戈纳巴德医院转诊患者中大肠埃希菌致病基因（traT、hly、aer、pap 和 fimH）和抗生素耐药基因（blaTEM、blaSHV 和 blaCTX）的鉴定与评估。

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2023-10-01 DOI:10.61186/rbmb.12.3.465

Alireza Mohammadzadeh, Hamid Naghizadeh, Ahmad Mosadegh, Akram Astani, Omid Pouresmaeil, Jalal Mardaneh

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引用次数: 0

摘要

背景：尿路感染（UTI）是常见的细菌感染之一。大肠埃希菌是导致尿路感染的最常见原因。本研究调查了几种毒力因子和β-内酰胺耐药基因的流行情况：方法：从转诊至 Allame-Bohlol Gonabadi 医院的UTI 患者标本中收集了 100 份大肠杆菌分离物。聚合酶链反应（PCR）鉴定了五个致病基因（fimH、aer、pap、hly、traT）和三个抗生素耐药基因（blaTEM、blaCTX、blaSHV）：结果：在扩谱β-内酰胺酶（ESBLs）阳性分离物中，blaSHV、blaTEM和blaCTXβ-内酰胺酶基因的频率分别为11.1%、48.1%和93.3%。大量分离菌株对最常用的抗生素具有耐药性：结论：致病基因也可能增加尿路感染的严重性、恶化和扩大。结论：致病基因也可能会增加尿路感染的严重程度、恶化和扩大，因此，确定这些基因是疾病的关键控制因子，可以更好地进行治疗。

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Identification and Evaluation of Pathogenic Genes (traT, hly, aer, pap, and fimH) and Antibiotic Resistance Genes (blaTEM, blaSHV, and blaCTX) in Escherichia coli in Patients Referred to Gonabad Hospitals, Iran.

Background: Urinary tract infection (UTI) is one of the common bacterial infections. Escherichia coli is the most common cause of UTI. In this research, the prevalence of several virulence factors and beta-lactam resistance genes was investigated.

Methods: One hundred E. coli isolates were collected from patients' specimens with UTI referred to Allame-Bohlol Gonabadi hospital. Polymerase chain reaction (PCR) was performed to identify five pathogenic genes (fimH, aer, pap, hly, traT) and three antibiotic resistance genes (blaTEM, blaCTX, blaSHV).

Results: The frequencies of blaSHV, blaTEM and blaCTX beta-lactamase genes among extended-spectrum-beta-lactamases (ESBLs) positive isolates were 11.1%, 48.1%, and 93.3%, respectively. A significant number of isolates were resistant to the most commonly used antibiotics.

Conclusion: Pathogenic genes may also increase the severity, progression, and expansion of urinary tract infections. Therefore, identifying these genes as critical controllers of illness can use for better manage the treatment.

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来源期刊

Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

2.80

自引率

23.50%

发文量

审稿时长

10 weeks

期刊介绍： The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.