人体牙本质界面的三维体外模型显示了牙本质表面的长程骨诱导作用

IF 10.8 1区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE International Journal of Oral Science Pub Date : 2024-05-11 DOI:10.1038/s41368-024-00298-9
William Macalester, Asme Boussahel, Rafael O. Moreno-Tortolero, Mark R. Shannon, Nicola West, Darryl Hill, Adam Perriman
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引用次数: 0

摘要

针对牙槽骨缺失的新兴再生细胞疗法已开始探索使用含细胞的水凝胶进行微创手术,以治疗小型和空间复杂的上颌骨-口腔缺损。然而,口腔为体内骨组织工程学提供了一个独特而具有挑战性的环境,它同时表现出牙周软硬组织,也是许多不同微生物群落的关键生物栖息地,这些微生物群落与外部环境和体内系统相互作用,这将影响细胞的命运和随后的治疗效果。在此,我们设计并生物打印了一个简便的人体牙本质界面三维体外模型,以探究牙本质表面对包裹在微孔水凝胶生物墨水中的人体间充质干细胞(hMSCs)的影响。我们证明,牙本质基底可诱导封装的 hMSCs 成骨分化,而且牙本质和β-磷酸三钙基底都能刺激凝胶-介质界面(即凝胶-基底界面的远端)上细胞外基质的生成和成熟。我们的研究结果表明,在骨组织工程过程中,矿化表面可能对干细胞产生长程影响,并为快速开发三维牙-骨界面模型提供了一个框架。
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A 3D In-vitro model of the human dentine interface shows long-range osteoinduction from the dentine surface

Emerging regenerative cell therapies for alveolar bone loss have begun to explore the use of cell laden hydrogels for minimally invasive surgery to treat small and spatially complex maxilla-oral defects. However, the oral cavity presents a unique and challenging environment for in vivo bone tissue engineering, exhibiting both hard and soft periodontal tissue as well as acting as key biocenosis for many distinct microbial communities that interact with both the external environment and internal body systems, which will impact on cell fate and subsequent treatment efficacy. Herein, we design and bioprint a facile 3D in vitro model of a human dentine interface to probe the effect of the dentine surface on human mesenchymal stem cells (hMSCs) encapsulated in a microporous hydrogel bioink. We demonstrate that the dentine substrate induces osteogenic differentiation of encapsulated hMSCs, and that both dentine and β-tricalcium phosphate substrates stimulate extracellular matrix production and maturation at the gel-media interface, which is distal to the gel-substrate interface. Our findings demonstrate the potential for long-range effects on stem cells by mineralized surfaces during bone tissue engineering and provide a framework for the rapid development of 3D dentine-bone interface models.

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来源期刊
International Journal of Oral Science
International Journal of Oral Science DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
31.80
自引率
1.30%
发文量
53
审稿时长
>12 weeks
期刊介绍: The International Journal of Oral Science covers various aspects of oral science and interdisciplinary fields, encompassing basic, applied, and clinical research. Topics include, but are not limited to: Oral microbiology Oral and maxillofacial oncology Cariology Oral inflammation and infection Dental stem cells and regenerative medicine Craniofacial surgery Dental material Oral biomechanics Oral, dental, and maxillofacial genetic and developmental diseases Craniofacial bone research Craniofacial-related biomaterials Temporomandibular joint disorder and osteoarthritis The journal publishes peer-reviewed Articles presenting new research results and Review Articles offering concise summaries of specific areas in oral science.
期刊最新文献
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