T A Semashko, G Y Fisunov, E A Tsoy, D R Kharrasov, I K Chudinov, D V Evsyutina, G Y Shevelev, V M Govorun
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引用次数: 0
摘要
在合成生物学的许多任务中，DNA 片段组装方法的标准化至关重要。这对于合成更广泛的序列库以及进一步实现此类反应的自动化和微型化都是必要的。在这项工作中，我们提出了用化学合成的寡核苷酸组装 DNA 片段的条件，并确定了在这些条件下序列中出现的错误。此外，我们还提出了将合成片段进一步组合成更大 DNA 片段的条件。我们的研究表明，优化后的条件适用于多种序列的组装。
Modern Approaches to de novo Synthesis of Extended DNA Fragments: Assembly of a Wide Repertoire of Sequences.
The standardization of DNA fragment assembly methods for many tasks of synthetic biology is crucial. This is necessary for synthesizing a wider repertoire of sequences, as well as for further automation and miniaturization of such reactions. In this work, we proposed conditions for the assembly of DNA fragments from chemically synthesized oligonucleotides and we identified the errors occurring in the sequence under these conditions. Additionally, we proposed conditions for further combining synthetic fragments into larger DNA fragments. We showed that the optimized conditions are suitable for the assembly of a wide range of sequences.
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