{"title":"用于硫醇、二硫化物和硫代酯的稳定而灵敏的 TLC 喷雾剂","authors":"Paul P. Van Veldhoven","doi":"10.1007/s10337-024-04336-w","DOIUrl":null,"url":null,"abstract":"<div><p>A sensitive and stable spray for thin layer chromatography of acyl-CoA and other thio-compounds has been evaluated. CoA, down to 1 nmol, is readily revealed and by pre- or post-treatment with hydroxylamine or tris(2-carboxyethyl)phosphine, thioesters or disulfides respectively can be stained as well.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 6","pages":"445 - 449"},"PeriodicalIF":1.2000,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Stable and Sensitive TLC-Spray for Thiols, Disulfides and Thioesters\",\"authors\":\"Paul P. Van Veldhoven\",\"doi\":\"10.1007/s10337-024-04336-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A sensitive and stable spray for thin layer chromatography of acyl-CoA and other thio-compounds has been evaluated. CoA, down to 1 nmol, is readily revealed and by pre- or post-treatment with hydroxylamine or tris(2-carboxyethyl)phosphine, thioesters or disulfides respectively can be stained as well.</p></div>\",\"PeriodicalId\":518,\"journal\":{\"name\":\"Chromatographia\",\"volume\":\"87 6\",\"pages\":\"445 - 449\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2024-05-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chromatographia\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10337-024-04336-w\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chromatographia","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s10337-024-04336-w","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
摘要
一种用于酰基-CoA 和其他硫代化合物薄层色谱的灵敏而稳定的喷雾器已通过评估。低至 1 nmol 的 CoA 很容易被显示出来，而且通过羟胺或三(2-羧乙基)膦的预处理或后处理，硫酯或二硫化物也可分别被染色。
A Stable and Sensitive TLC-Spray for Thiols, Disulfides and Thioesters
A sensitive and stable spray for thin layer chromatography of acyl-CoA and other thio-compounds has been evaluated. CoA, down to 1 nmol, is readily revealed and by pre- or post-treatment with hydroxylamine or tris(2-carboxyethyl)phosphine, thioesters or disulfides respectively can be stained as well.
期刊介绍：
Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
