{"title":"评估西布曲霉对 CCl4 引起的大鼠肝损伤的保肝作用","authors":"Amirhossein Sarkar, Omid Sabzevari, Seyed Esmaeil Sadat Ebrahimi, Sana Shahnazari, Reza Asadollahi Rad, Mahdi Vazirian","doi":"10.1615/intjmedmushrooms.2024053967","DOIUrl":null,"url":null,"abstract":"Mushrooms have been used as medicine by humans for more than 5,000 years. Nowadays, some extracts and compounds obtained from medicinal mushrooms have increased a great prospect of treating many disorders by having a great role in modulation of immune system, cancer inhibiting, cardio-vascular health, antiviral, antibacterial, antioxidant and protective effects against hepatitis and diabetes.\nIn this study, we evaluated the antioxidant effect of methanol and hot water extract of the Trametes gibbosa (Pers.) Fr. mushroom and hepatoprotective effect of the extract with the most radical scavenging potency. To assess the antioxidant properties of different extracts of the mushroom, DPPH method was used. For assessing the hepatoprotective properties, a seven-day experiment was designed and liver toxicity was induced by carbon tetrachloride (intraperitoneal/ip for seven consecutive days, 0.5 ml/kg BW). Rats were simultaneously fed with aqueous extract of the mushroom with the dose of 250, 500 and 1000 mg/kg BW and silymarin (100 mg/kg BW) as positive control. At the end of the experiment, major liver factors (e.g., AST, ALT, ALP, bilirubin) were quantified. Tissue samples were obtained for pathological examination.\nAqueous extract showed more potent radical scavenging activity (IC50=414.33 µg/ml, compared to 936.92 µg/ml for methanolic extract). Indeed, hepatoprotective properties of the aqueous extract of the mushroom were comparable with those of silymarin and even showed superior protective effects in histopathological examination. It seems that with further complementary studies, T. gibbosa could be considered a potential candidate for hepatoprotection.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of hepatoprotective effect of Trametes gibbosa on CCl4-induced liver injury in rats\",\"authors\":\"Amirhossein Sarkar, Omid Sabzevari, Seyed Esmaeil Sadat Ebrahimi, Sana Shahnazari, Reza Asadollahi Rad, Mahdi Vazirian\",\"doi\":\"10.1615/intjmedmushrooms.2024053967\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Mushrooms have been used as medicine by humans for more than 5,000 years. Nowadays, some extracts and compounds obtained from medicinal mushrooms have increased a great prospect of treating many disorders by having a great role in modulation of immune system, cancer inhibiting, cardio-vascular health, antiviral, antibacterial, antioxidant and protective effects against hepatitis and diabetes.\\nIn this study, we evaluated the antioxidant effect of methanol and hot water extract of the Trametes gibbosa (Pers.) Fr. mushroom and hepatoprotective effect of the extract with the most radical scavenging potency. To assess the antioxidant properties of different extracts of the mushroom, DPPH method was used. For assessing the hepatoprotective properties, a seven-day experiment was designed and liver toxicity was induced by carbon tetrachloride (intraperitoneal/ip for seven consecutive days, 0.5 ml/kg BW). Rats were simultaneously fed with aqueous extract of the mushroom with the dose of 250, 500 and 1000 mg/kg BW and silymarin (100 mg/kg BW) as positive control. At the end of the experiment, major liver factors (e.g., AST, ALT, ALP, bilirubin) were quantified. Tissue samples were obtained for pathological examination.\\nAqueous extract showed more potent radical scavenging activity (IC50=414.33 µg/ml, compared to 936.92 µg/ml for methanolic extract). Indeed, hepatoprotective properties of the aqueous extract of the mushroom were comparable with those of silymarin and even showed superior protective effects in histopathological examination. It seems that with further complementary studies, T. gibbosa could be considered a potential candidate for hepatoprotection.\",\"PeriodicalId\":14025,\"journal\":{\"name\":\"International journal of medicinal mushrooms\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2024-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of medicinal mushrooms\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1615/intjmedmushrooms.2024053967\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of medicinal mushrooms","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1615/intjmedmushrooms.2024053967","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MYCOLOGY","Score":null,"Total":0}
Evaluation of hepatoprotective effect of Trametes gibbosa on CCl4-induced liver injury in rats
Mushrooms have been used as medicine by humans for more than 5,000 years. Nowadays, some extracts and compounds obtained from medicinal mushrooms have increased a great prospect of treating many disorders by having a great role in modulation of immune system, cancer inhibiting, cardio-vascular health, antiviral, antibacterial, antioxidant and protective effects against hepatitis and diabetes.
In this study, we evaluated the antioxidant effect of methanol and hot water extract of the Trametes gibbosa (Pers.) Fr. mushroom and hepatoprotective effect of the extract with the most radical scavenging potency. To assess the antioxidant properties of different extracts of the mushroom, DPPH method was used. For assessing the hepatoprotective properties, a seven-day experiment was designed and liver toxicity was induced by carbon tetrachloride (intraperitoneal/ip for seven consecutive days, 0.5 ml/kg BW). Rats were simultaneously fed with aqueous extract of the mushroom with the dose of 250, 500 and 1000 mg/kg BW and silymarin (100 mg/kg BW) as positive control. At the end of the experiment, major liver factors (e.g., AST, ALT, ALP, bilirubin) were quantified. Tissue samples were obtained for pathological examination.
Aqueous extract showed more potent radical scavenging activity (IC50=414.33 µg/ml, compared to 936.92 µg/ml for methanolic extract). Indeed, hepatoprotective properties of the aqueous extract of the mushroom were comparable with those of silymarin and even showed superior protective effects in histopathological examination. It seems that with further complementary studies, T. gibbosa could be considered a potential candidate for hepatoprotection.
期刊介绍:
The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.