Jungae Lim, Jin-Hyuk Lim, Ji-Hoon Lee, Su-Hwan Cheon, Guewha Lee, Z-Hun Kim, Dong-Il Kim
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引用次数: 0
摘要
中国仓鼠卵巢（CHO）细胞在生物技术产业中被广泛用于生产治疗蛋白，从而引发了提高治疗蛋白生产率的各种研究工作。虽然在 CHO 细胞培养过程中添加小分子增强剂可以提高生产率，但也会对产品质量产生负面影响。本研究调查了 4-苯基丁酸盐（4-PBA）的影响，4-苯基丁酸盐是一种化学伴侣素，对 CHO 细胞中重组蛋白的生产有积极影响。通过改变添加 4-PBA 的时间，证实滴度可提高 1.57 倍，同时减少了生长抑制和细胞凋亡等负面影响，从而保证了产品质量。4-PBA 的这些作用证明了使用添加剂开发用于生产重组蛋白的新型增强剂和制定 CHO 细胞工程策略的潜力。
Effect of 4-phenylbutyrate addition timing on titer of Fc-fusion protein in Chinese hamster ovary cell cultures
Chinese hamster ovary (CHO) cells have been widely used in the biotechnology industry for the production of therapeutic proteins, leading to various research efforts to increase the productivity of therapeutic proteins. Although the addition of small molecule enhancers in CHO cell culture can enhance productivity, it poses a problem by negatively affecting product quality. In this study, effects of 4-phenylbutyrate (4-PBA), a chemical chaperone having a positive influence on recombinant protein production in CHO cells, were investigated. By varying the timing of 4-PBA addition, it was confirmed that the titer could be increased by 1.57-fold while reducing negative effects such as growth inhibition and apoptosis, thus preserving product quality. These effects of 4-PBA demonstrate the potential of using additive for developing novel enhancers for the production of recombinant proteins and the development of strategies for CHO cell engineering.
期刊介绍：
Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
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