Weihong Fu, Lin Zhao, Wanjun Qiu, Xu Xu, Meng Ding, Liming Lan, Shenchun Qu, Sanhong Wang
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引用次数: 0
摘要
可溶性糖和有机酸是决定水果感官品质的关键因素,直接影响水果作物的商品价值和经济收益。我们对苹果品种 "嘎啦 "和 "小红楼 "及其 F1 代杂交种进行了全基因组测序(WGS),构建了高密度的基因组图谱。我们的定量遗传分析确定了与 11 个糖和酸性状相关的 53 个定量性状位点(QTL)。我们在连接组(LG)15的稳定QTL上发现了一个负责苹果酸降解的候选基因MdNADP-ME。序列分析显示,启动子区域(MEp-799)的一个 A/C SNP 影响转录因子 MdMYB2 的结合,从而影响 MdNADP-ME 的表达。在我们对各种苹果基因型的研究中,该 SNP 与苹果酸和果糖水平有关。我们还开发了一种与果实果糖含量相关的 dCAPS 标记。这些结果证实了 MdNADP-ME 在维持苹果果实中糖和酸含量平衡方面的作用。
Whole-genome resequencing identifies candidate genes and allelic variation in the MdNADP-ME promoter that regulate fruit malate and fructose contents in apple.
Soluble sugar and organic acids are key determinants of fruit organoleptic quality and directly affect the commodity value and economic returns of fruit crops. We performed whole-genome sequencing of the apple varieties Gala and Xiahongrou, along with their F1 hybrids, to construct a high-density bin map. Our quantitative genetic analysis pinpointed 53 quantitative trait loci (QTLs) related to 11 sugar and acid traits. We identified a candidate gene, MdNADP-ME, responsible for malate degradation, in a stable QTL on linkage group 15. Sequence analysis revealed an A/C SNP in the promoter region (MEp-799) that influences binding of the MdMYB2 transcription factor, thereby affecting MdNADP-ME expression. In our study of various apple genotypes, this SNP has been demonstrated to be linked to malate and fructose levels. We also developed a dCAPS marker associated with fruit fructose content. These results substantiate the role of MdNADP-ME in maintaining the equilibrium between sugar and acid contents in apple fruits.
期刊介绍:
Plant Communications is an open access publishing platform that supports the global plant science community. It publishes original research, review articles, technical advances, and research resources in various areas of plant sciences. The scope of topics includes evolution, ecology, physiology, biochemistry, development, reproduction, metabolism, molecular and cellular biology, genetics, genomics, environmental interactions, biotechnology, breeding of higher and lower plants, and their interactions with other organisms. The goal of Plant Communications is to provide a high-quality platform for the dissemination of plant science research.