Elisa Albertini, Stefano Chillotti, Giada Monti, Deborah Malvi, Marzia Deserti, Alessio Degiovanni, Andrea Palloni, Simona Tavolari, Giovanni Brandi, Antonia D'Errico, Francesco Vasuri
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Histology was revised in all cases, and albumin ISH was automatically performed by the RNAscope®.</p><p><strong>Results: </strong>ISH was always negative in extrahepatic CCAs, only 1 perihilar case was positive, and any positivity was observed in 25/29 (86.2%) intrahepatic CCAs (p < 0.001). Concerning CCA subtypes, mean cell positivity was 38.8 ± 29.8% in small-duct CCAs and 11.4 ± 21.9 in large-duct CCAs, respectively (p = 0.003); 12/15 (80.0%) small-duct and 3/14 (21.4%) large-duct CCAs showed >5% positive cells (p = 0.002; odds ratio 14.7).</p><p><strong>Conclusions: </strong>The introduction of more sensitive techniques changed the indications for ISH since most small-duct intrahepatic CCAs show diffuse positivity. 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引用次数: 0
摘要
简介近年来,白蛋白原位杂交(ISH)技术的进步改变了肝脏恶性肿瘤的常规应用和鉴别诊断。本研究旨在评估白蛋白原位杂交对不同胆管癌(CCA)亚型的诊断效用,并评估白蛋白生成在胆道树上的变化情况:回顾性筛选出 45 例 CCA:29 例肝内(15 例小导管亚型和 14 例大导管亚型)、7 例肝周和 9 例肝外。所有病例都进行了组织学检查,并由 RNAscope® 自动进行白蛋白 ISH:结果:肝外CCA的白蛋白ISH始终为阴性,只有1例肝周阳性,25/29(86.2%)例肝内CCA观察到阳性细胞(p5%阳性细胞,p=0.002;奇异比率14.7):结论:灵敏度更高的技术的引入改变了ISH的适应症,因为大多数小导管肝内CCA呈现弥漫性阳性。白蛋白阳性率从肝周边到大导管逐渐降低,这表明 ISH 可以帮助鉴别诊断小导管和大导管 CCA,以及肝内大导管 CCA 和转移瘤。
Decreasing Albumin mRNA Expression in Cholangiocarcinomas along the Bile Duct Tree.
Introduction: The progressive technologies in albumin in situ hybridization (ISH) changed the routine application and the differential diagnosis of hepatic malignancies in the last years. The aim of the present work was to assess the diagnostic utility of albumin ISH on different cholangiocarcinoma (CCA) subtypes, as well as to assess how albumin production changes along the biliary tree.
Methods: Forty-five CCAs were retrospectively selected: 29 intrahepatic (15 small-duct and 14 large-duct subtypes), 7 perihilar, and 9 extrahepatic. Histology was revised in all cases, and albumin ISH was automatically performed by the RNAscope®.
Results: ISH was always negative in extrahepatic CCAs, only 1 perihilar case was positive, and any positivity was observed in 25/29 (86.2%) intrahepatic CCAs (p < 0.001). Concerning CCA subtypes, mean cell positivity was 38.8 ± 29.8% in small-duct CCAs and 11.4 ± 21.9 in large-duct CCAs, respectively (p = 0.003); 12/15 (80.0%) small-duct and 3/14 (21.4%) large-duct CCAs showed >5% positive cells (p = 0.002; odds ratio 14.7).
Conclusions: The introduction of more sensitive techniques changed the indications for ISH since most small-duct intrahepatic CCAs show diffuse positivity. Albumin positivity decreases from liver periphery to the large ducts, suggesting that ISH can be helpful in the differential diagnosis between small-duct and large-duct CCAs, as well as between intrahepatic large-duct CCAs and metastases.
期刊介绍:
''Pathobiology'' offers a valuable platform for the publication of high-quality original research into the mechanisms underlying human disease. Aiming to serve as a bridge between basic biomedical research and clinical medicine, the journal welcomes articles from scientific areas such as pathology, oncology, anatomy, virology, internal medicine, surgery, cell and molecular biology, and immunology. Published bimonthly, ''Pathobiology'' features original research papers and reviews on translational research. The journal offers the possibility to publish proceedings of meetings dedicated to one particular topic.