M. Reyzov, M. Eftimov, S. Gancheva, M. Todorova, M. Zhelyazkova-Savova, M. Tzaneva, S. Valcheva-Kuzmanova
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引用次数: 0
摘要
本研究评估了在高脂高果糖饮食(HFHF)诱导的代谢综合征(MS)模型中,Aronia melanocarpa果汁(AMFJ)对肝脏的影响。50 只大鼠被分为五组--对照组、MS 组、MS+AMFJ2.5 组、MS+AMFJ5 组和 MS+AMFJ10 组。对照组喂食标准饮食,其他组喂食高氟高纤维素饮食。在 MS 诱导期间,每天进行口服治疗。对照组和 MS 组接受 10.0 mL kg-1 的蒸馏水,其他三组分别接受 2.5、5.0 和 10.0 mL kg-1 剂量的 AMFJ。10 周后,收集肝脏样本并进行组织学和免疫组化检查,以确定 Bax、Bcl-2 和 MAC387 的表达。计算 Bax/Bcl-2 比率。在多发性硬化大鼠的肝脏中发现了脂肪变性、炎症和退行性改变,Bax 和 MAC387 明显升高,而 Bcl-2 则无明显降低。Bax/Bcl-2 比值明显升高。AMFJ 治疗阻止了组织病理学的改变。与 MS 组相比,AMFJ 治疗组的 Bax 和 MAC387 值明显降低,Bcl-2 值升高,导致 Bax/Bcl-2 比值明显降低(P < 0.001)。在 MS 诱导期间给大鼠注射 AMFJ 可防止肝脏发生炎症、脂肪变性、退行性和促凋亡变化。
Aronia melanocarpa fruit juice prevents hepatic impairment in rats subjected to metabolic syndrome
This study evaluated the effects of Aronia melanocarpa fruit juice (AMFJ) on the liver in a model of high-fat, high-fructose (HFHF) diet-induced metabolic syndrome (MS). Fifty rats were allocated to five groups – Control, MS, MS+AMFJ2.5, MS+AMFJ5, and MS+AMFJ10. The control group was fed a standard diet, while the other groups were provided a HFHF diet. During MS induction, daily oral treatment was performed. Control and MS groups received 10.0 mL kg−1 distilled water, while the other three groups received AMFJ at doses of 2.5, 5.0, and 10.0 mL kg−1, respectively. After 10 weeks, liver samples were collected and inspected histologically as well as immunohistochemically to determine the expression of Bax, Bcl-2, and MAC387. Bax/Bcl-2 ratio was calculated. In MS rats, steatotic, inflammatory and degenerative alterations of the liver were detected, Bax and MAC387 were markedly elevated, while Bcl-2 was non-significantly reduced. The Bax/Bcl-2 ratio significantly increased. The histopathological alterations were prevented by the AMFJ treatment. Compared to MS group, Bax and MAC387 values were significantly lower and Bcl-2 value was higher resulting in significantly lower (P < 0.001) Bax/Bcl-2 ratio in all AMFJ-treated groups. AMFJ, administered during MS induction in rats, prevented the occurrence of inflammatory, steatotic, degenerative, and pro-apoptotic changes in the liver.
期刊介绍:
Acta Alimentaria publishes original papers and reviews on food science (physics, physical chemistry, chemistry, analysis, biology, microbiology, enzymology, engineering, instrumentation, automation and economics of foods, food production and food technology, food quality, post-harvest treatments, food safety and nutrition).