波形蛋白通过增强中心体功能和微管乙酰化来支持细胞极化。

IF 3.7 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Journal of The Royal Society Interface Pub Date : 2024-06-01 Epub Date: 2024-06-05 DOI:10.1098/rsif.2023.0641
Renita Saldanha, Minh Tri Ho Thanh, Nikhila Krishnan, Heidi Hehnly, Alison Patteson
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引用次数: 0

摘要

细胞极性对于控制细胞形状、运动和细胞分裂过程非常重要。波形蛋白中间丝对间充质细胞的细胞迁移和细胞极化非常重要,波形蛋白和微管网络的组装是动态协调的,但波形蛋白如何介导细胞极性的确切细节仍不清楚。在这里,我们描述了波形蛋白对野生型和波形蛋白缺失型小鼠胚胎成纤维细胞中心体的结构和功能以及微管丝稳定性的影响。我们发现,波形蛋白介导了包膜材料的结构,促进了中心体介导的微管再生,并增加了细胞中稳定乙酰化微管的水平。在伤口闭合过程中发生的细胞极化和迁移过程中,波形蛋白的缺失也会影响中心体的重新定位。我们的研究结果表明,波形蛋白可调节中心体的结构和功能以及微管网络的稳定性,这对细胞如何建立正确的细胞极化和持续迁移具有重要意义。
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Vimentin supports cell polarization by enhancing centrosome function and microtubule acetylation.

Cell polarity is important for controlling cell shape, motility and cell division processes. Vimentin intermediate filaments are important for cell migration and cell polarization in mesenchymal cells and assembly of vimentin and microtubule networks is dynamically coordinated, but the precise details of how vimentin mediates cell polarity remain unclear. Here, we characterize the effects of vimentin on the structure and function of the centrosome and the stability of microtubule filaments in wild-type and vimentin-null mouse embryonic fibroblasts. We find that vimentin mediates the structure of the pericentriolar material, promotes centrosome-mediated microtubule regrowth and increases the level of stable acetylated microtubules in the cell. Loss of vimentin also impairs centrosome repositioning during cell polarization and migration processes that occur during wound closure. Our results suggest that vimentin modulates centrosome structure and function as well as microtubule network stability, which has important implications for how cells establish proper cell polarization and persistent migration.

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来源期刊
Journal of The Royal Society Interface
Journal of The Royal Society Interface 综合性期刊-综合性期刊
CiteScore
7.10
自引率
2.60%
发文量
234
审稿时长
2.5 months
期刊介绍: J. R. Soc. Interface welcomes articles of high quality research at the interface of the physical and life sciences. It provides a high-quality forum to publish rapidly and interact across this boundary in two main ways: J. R. Soc. Interface publishes research applying chemistry, engineering, materials science, mathematics and physics to the biological and medical sciences; it also highlights discoveries in the life sciences of relevance to the physical sciences. Both sides of the interface are considered equally and it is one of the only journals to cover this exciting new territory. J. R. Soc. Interface welcomes contributions on a diverse range of topics, including but not limited to; biocomplexity, bioengineering, bioinformatics, biomaterials, biomechanics, bionanoscience, biophysics, chemical biology, computer science (as applied to the life sciences), medical physics, synthetic biology, systems biology, theoretical biology and tissue engineering.
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