肌肽和染料木素的组合可抑制 MCF-7 人类乳腺癌细胞的癌症干性。

IF 2 4区 医学 Q3 NUTRITION & DIETETICS Nutrition Research and Practice Pub Date : 2024-06-01 Epub Date: 2024-05-10 DOI:10.4162/nrp.2024.18.3.436
Hyeji Kwon, Yuri Kim, Jung Hyun Kim
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引用次数: 0

摘要

背景/目的:乳腺癌在全世界都被视为一个严重的健康问题,并受到包括缺乏运动和不健康饮食在内的风险因素的影响。肌肉在体力活动中分泌的肌动素在癌症的发展和免疫系统中起着至关重要的作用。染料木素(Genistein,Gen)是一种主要存在于豆类中的异黄酮,可通过调节癌症干细胞(CSCs)诱导抗癌活性。因此,本研究探讨了肌肽和 Gen 组合对人类乳腺癌 MCF-7 细胞的潜在抗癌作用:材料/方法:体外研究使用了人乳腺癌细胞株 MCF-7。使用 MTT 试验评估了 MCF-7 细胞在肌动素、鸢尾素(Iri)、oncostatin M(OSM)和 Gen 处理后的存活率。克隆形成和球形成试验用于评估乳腺癌干细胞的自我更新能力。对MCF-7乳腺癌细胞中干细胞标志物的mRNA表达水平进行了分析:结果:给予Iri或OSM与Gen能显著抑制MCF-7细胞的自我更新能力。此外,乳腺癌干细胞标志物SOX2和OCT4的mRNA表达也受到myokine和Gen的抑制,但将Iri或OSM与Gen联合使用是最有效的治疗方法:这些结果表明,将 Iri 或 OSM 与 Gen 结合使用可通过调节自我更新能力和 CSCs 标志物的表达对乳腺癌 CSCs 产生叠加效应。因此,肌动蛋白与 Gen 的联合治疗可能具有治疗乳腺癌和改善癌症患者生活质量的潜力。
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A combination of myokines and genistein suppresses cancer stemness in MCF-7 human breast cancer cells.

Background/objectives: Breast cancer is considered a serious health issue worldwide and is influenced by risk factors, including physical inactivity and unhealthy diet. Myokines secreted by muscles during physical activity play a crucial role in cancer development and the immune system. Genistein (Gen), an isoflavone primarily in legumes, induces anti-cancer activity by regulating cancer stem cells (CSCs). Therefore, this study investigated the potential anti-cancer effect of a combination of myokine and Gen on the human breast cancer MCF-7 cells.

Materials/methods: MCF-7, a human breast cancer cell line, was used for in vitro study. The cell viability of MCF-7 cells was evaluated in response to treatment with myokines, irisin (Iri), oncostatin M (OSM), and Gen using the MTT assay. Clonogenic and sphere formation assays were used to evaluate the self-renewal capacity of breast CSCs. The mRNA expression levels of stem cell markers were analyzed in MCF-7 breast cancer cells.

Results: Administering Iri or OSM with Gen significantly inhibited the self-renewal capacity of MCF-7 cells. In addition, mRNA expression of breast CSC markers SOX2 and OCT4, which are characteristic of CSCs, was suppressed by both myokine and Gen. However, combining Iri or OSM with Gen was the most effective treatment.

Conclusion: These results suggested that combining Iri or OSM with Gen has an additive effect on breast CSCs by regulating self-renewal capacity and expression of CSCs markers. Therefore, the combination of myokines and Gen may have the therapeutic potential for treating breast cancer and improving the quality of life of cancer patients.

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来源期刊
Nutrition Research and Practice
Nutrition Research and Practice NUTRITION & DIETETICS-
CiteScore
3.50
自引率
4.20%
发文量
62
审稿时长
6-12 weeks
期刊介绍: Nutrition Research and Practice (NRP) is an official journal, jointly published by the Korean Nutrition Society and the Korean Society of Community Nutrition since 2007. The journal had been published quarterly at the initial stage and has been published bimonthly since 2010. NRP aims to stimulate research and practice across diverse areas of human nutrition. The Journal publishes peer-reviewed original manuscripts on nutrition biochemistry and metabolism, community nutrition, nutrition and disease management, nutritional epidemiology, nutrition education, foodservice management in the following categories: Original Research Articles, Notes, Communications, and Reviews. Reviews will be received by the invitation of the editors only. Statements made and opinions expressed in the manuscripts published in this Journal represent the views of authors and do not necessarily reflect the opinion of the Societies.
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