Yue Li, Rong Chang, Yang-Jia Liu, Feng Chen and Yong-Xiang Chen
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引用次数: 0
摘要
脱矿釉质的再生对治疗龋齿具有重要意义。釉质素(Ame)是介导釉质自然生长的一种重要蛋白质,在儿童期釉质完全成熟后就不再分泌。尽管基于多肽或蛋白质的生物仿矿化技术已取得重大进展,但目前仍缺乏易于获得、成本低廉、生物相容性好且高效的釉质素模拟物。在本文中,我们通过将 Ame 的 C 端与聚(γ-苄基-L-谷氨酸)轻松偶联,构建了一系列两亲性支链多肽(CAMPs),后者可模拟 Ame 的疏水性 N 端。其中,CAMP15 是最佳的仿生矿化模板,具有良好的自组装性能,可引导羟基磷灰石定向结晶,并能抑制变异链球菌和金黄色葡萄球菌在釉质表面的粘附。这项工作凸显了两亲性支链多肽作为 Ame 模拟物在修复缺损釉质方面的潜在应用,为龋齿的预防和治疗提供了一种前景广阔的策略。
Self-assembled branched polypeptides as amelogenin mimics for enamel repair†
The regeneration of demineralized enamel holds great significance in the treatment of dental caries. Amelogenin (Ame), an essential protein for mediating natural enamel growth, is no longer secreted after enamel has fully matured in childhood. Although biomimetic mineralization based on peptides or proteins has made significant progress, easily accessible, low-cost, biocompatible and highly effective Ame mimics are still lacking. Herein, we construct a series of amphiphilic branched polypeptides (CAMPs) by facile coupling of the Ame's C-terminal segment and poly(γ-benzyl-L-glutamate), which serves to simulate the Ame's hydrophobic N-terminal segment. Among them, CAMP15 is the best biomimetic mineralization template with great self-assembly performance to guide the oriented crystallization of hydroxyapatite and is capable of inhibiting the adhesion of Streptococcus mutans and Staphylococcus aureus on the enamel surfaces. This work highlights the potential application of amphiphilic branched polypeptide as Ame mimics in repairing defected enamel, providing a promising strategy for prevention and treatment of dental caries.
期刊介绍:
Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive:
Antifouling coatings
Biocompatible materials
Bioelectronics
Bioimaging
Biomimetics
Biomineralisation
Bionics
Biosensors
Diagnostics
Drug delivery
Gene delivery
Immunobiology
Nanomedicine
Regenerative medicine & Tissue engineering
Scaffolds
Soft robotics
Stem cells
Therapeutic devices