日本一家未明显爆发耐万古霉素肠球菌疫情的医院从住院病人中分离的肠球菌的抗生素敏感性和基因组分析。

IF 1.9 4区 医学 Q4 IMMUNOLOGY Microbiology and Immunology Pub Date : 2024-06-14 DOI:10.1111/1348-0421.13155
Ayumi Fujii, Miki Kawada-Matsuo, Mi Nguyen-Tra Le, Kanako Masuda, Kayoko Tadera, Yujin Suzuki, Saki Nishihama, Junzo Hisatsune, Yo Sugawara, Seiya Kashiyama, Hideki Shiba, Tomonao Aikawa, Hiroki Ohge, Motoyuki Sugai, Hitoshi Komatsuzawa
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引用次数: 0

摘要

为预防院内感染,必须筛查患者中可能存在的耐万古霉素肠球菌(VRE)。在本研究中,我们分析了一家未明显爆发 VRE 的医院中住院患者的肠球菌分离物。我们使用肠球菌选择性培养基收集了广岛大学医院 2021 年 4 月 1 日至 6 月 30 日住院患者的肠球菌分离物。进行了多焦点序列分型、抗菌药物药敏试验和全基因组测序。共分析了 164 个分离株,包括粪肠球菌(41 个分离株)、粪肠球菌(80 个分离株)、拉菲诺斯肠球菌(11 个分离株)、卡氏肠球菌(9 个分离株)、阿维氏肠球菌(12 个分离株)、乳酸肠球菌(8 个分离株)、加里纳氏肠球菌(2 个分离株)和恶臭肠球菌(1 个分离株)。我们发现了 1 株 vanA 阳性的粪肠球菌(患者转院时已获知)、9 株 vanC 阳性的卡氏肠球菌和 2 株 vanC 阳性的加里纳氏肠球菌。分离出的粪肠球菌对氨苄西林(95.1%)、亚胺培南(95.1%)和左氧氟沙星(87.8%)具有耐药性,对米诺环素(49.4%)具有耐药性。对氨苄西林和左氧氟沙星耐药的粪肠球菌分别对青霉素结合蛋白 5(PBP5)(39/39 个分离株)和 ParC/GyrA (21/36 个分离株)有多重突变。拉菲诺斯大肠杆菌对氨苄西林(81.8%)、亚胺培南(45.5%)和左氧氟沙星(45.5%)产生耐药性,乳酸杆菌对氨苄西林(37.5%)和亚胺培南(50.0%)产生耐药性。仅在粪大肠杆菌和拉菲诺斯大肠杆菌的一个分离株中分别发现了利奈唑胺耐药基因 optrA 和 cfr(B)。这项研究显示了住院患者肠球菌感染的状况,是考虑控制 VRE 的院内感染时的重要信息之一。
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Antibiotic susceptibility and genome analysis of Enterococcus species isolated from inpatients in one hospital with no apparent outbreak of vancomycin-resistant Enterococcus in Japan

To prevent nosocomial infection, it is important to screen for potential vancomycin-resistant Enterococcus (VRE) among patients. In this study, we analyzed enterococcal isolates from inpatients in one hospital without any apparent outbreak of VRE. Enterococcal isolates were collected from inpatients at Hiroshima University Hospital from April 1 to June 30, 2021 using selective medium for Enterococci. Multilocus sequence typing, antimicrobial susceptibility testing, and whole-genome sequencing were performed. A total of 164 isolates, including Enterococcus faecium (41 isolates), Enterococcus faecalis (80 isolates), Enterococcus raffinosus (11 isolates), Enterococcus casseliflavus (nine isolates), Enterococcus avium (12 isolates), Enterococcus lactis (eight isolates), Enterococcus gallinarum (two isolates), and Enterococcus malodoratus (one isolate), were analyzed. We found one vanA-positive E. faecium, which was already informed when the patient was transferred to the hospital, nine vanC-positive E. casseliflavus, and two vanC-positive E. gallinarum. E. faecium isolates showed resistance to ampicillin (95.1%), imipenem (95.1%), and levofloxacin (87.8%), and E. faecalis isolates showed resistance to minocycline (49.4%). Ampicillin- and levofloxacin-resistant E. faecium had multiple mutations in penicillin-binding protein 5 (PBP5) (39/39 isolates) and ParC/GyrA (21/36 isolates), respectively. E. raffinosus showed resistance to ampicillin (81.8%), imipenem (45.5%), and levofloxacin (45.5%), and E. lactis showed resistance to ampicillin (37.5%) and imipenem (50.0%). The linezolid resistance genes optrA and cfr(B) were found only in one isolate of E. faecalis and E. raffinosus, respectively. This study, showing the status of enterococci infection in hospitalized patients, is one of the important information when considering nosocomial infection control of VRE.

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来源期刊
Microbiology and Immunology
Microbiology and Immunology 医学-免疫学
CiteScore
5.20
自引率
3.80%
发文量
78
审稿时长
1 months
期刊介绍: Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.
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Issue Information – Cover A single amino acid substitution in the Borna disease virus glycoprotein enhances the infectivity titer of vesicular stomatitis virus pseudotyped virus by altering membrane fusion activity. Structure-based virtual screening and drug repurposing studies indicate potential inhibitors of bovine papillomavirus E6 oncoprotein. Downregulation of CD86 in HCMV-infected THP-1 cells. Issue Information – Cover
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