慢性子宫内膜炎。局部促氧化剂、抗氧化剂和免疫代谢紊乱。一项开放标签、前瞻性、随机研究

Q3 Medicine Gynecology Pub Date : 2024-06-10 DOI:10.26442/20795696.2024.2.202800
O. Gizinger, Y. Sorokin, V. Radzinskiy
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The activity of lipid peroxidation factors was analyzed: primary, secondary and final products of lipid peroxidation in heptane and isopropanolol fractions of uterine cavity aspirate, enzymes of the antioxidant system: superoxide dismutase, catalase, glutathione peroxidase, deoxyribonuclease I (DNase), by spectrophotometric method, remodeling factors – matrix metalloproteinases MMP-2, -9 by flow fluorimetry. \nResults. In patients of group 1, according to transvaginal echography of the pelvic organs, M-echo was 6.29±1.22 mm, in group 2 – 9.10±1.15 mm. In chronic endometritis, disruption of pro-oxidant and activity of antioxidant factors in the endometrium in women with chronic endometritis and a history of reproductive losses. 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引用次数: 0

摘要

目的确定慢性子宫内膜炎的脂过氧化过程、抗氧化防御、基质金属蛋白酶状态的局部特征。材料和方法。研究对象为经组织学和免疫组化证实患有慢性子宫内膜炎并有生育损失史的 80 名妇女(年龄为 29.33±6.71(最小-最大值 [24-36]))(第 1 组)。对照组包括 30 名经组织化学研究结果证实无慢性子宫内膜炎且无生育史的输卵管腹膜源性不孕患者,年龄为 30.11±6.9(最小值-最大值[24-37];第 2 组)。所有 130 名患者均接受了经阴道盆腔器官超声检查,并对子宫内膜样本进行了组织学和免疫组化检查。采用分光光度法分析了脂质过氧化因子的活性:子宫腔吸出物庚烷和异丙醇组分中脂质过氧化的初级、次级和最终产物;抗氧化系统酶:超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、脱氧核糖核酸酶 I(DNase);流式荧光测定法分析了重塑因子--基质金属蛋白酶 MMP-2、-9。结果根据盆腔器官经阴道超声检查,第1组患者的M-回声为6.29±1.22毫米,第2组为9.10±1.15毫米。在慢性子宫内膜炎中,患有慢性子宫内膜炎并有生育损失史的妇女子宫内膜中的促氧化因子和抗氧化因子的活性受到破坏。在酚类提取物的庚烷馏分中,脂质过氧化的初级产物(二烯共轭物)的浓度比对照组高12.98%,脂质过氧化的次级产物(酮二烯)的含量比对照组的值高15.45%,共轭三烯的含量比对照组的值高18.67%,这表明CE期间子宫内膜细胞膜的脂质过氧化增加。在异丙醇相中,检测到磷脂酰基残基的脂质过氧化产物。在脂质提取物的异丙醇馏分中,二烯共轭物的含量较高,酮二烯含量为 24.32%,共轭三烯含量为 26.14%。与第 2 组的指标相比,超氧化物歧化酶的活性提高了 23.98%,过氧化氢酶提高了 14.34%,谷胱甘肽过氧化物酶提高了 12.39%,DN 酶提高了 2.9 倍。结论慢性子宫内膜炎伴随着促氧化因子和抗氧化因子系统、组织重塑产物含量的变化。子宫内膜氧化应激的病理变化可能是白细胞浸润增加,脂质过氧化的初级、次级和最终产物、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、脱氧核糖核酸酶 I(DNase)、基质金属蛋白酶-2、-9 的表达失衡。
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Chronic endometritis. Local prooxidant, antioxidant and immunometabolic disorders. An open-label, prospective, randomized study
Aim. To determine local features of lipoperoxidation processes, antioxidant defense, state of matrix metalloproteinases in chronic endometritis. Materials and methods. Eighty women aged 29.33±6.71 (min-max [24–36]) with histologically and immunohistochemically confirmed chronic endometritis and a history of reproductive losses were examined (group 1). The control group consisted of 30 patients without chronic endometritis confirmed by the results of a histochemical study and no history of reproductive losses, suffering from infertility of tubal-peritoneal origin at the age of 30.11±6.9 years (min-max [24–37]; group 2). All 130 patients underwent transvaginal echography of the pelvic organs, and histological and immunohistochemical examination of endometrial samples was performed. The activity of lipid peroxidation factors was analyzed: primary, secondary and final products of lipid peroxidation in heptane and isopropanolol fractions of uterine cavity aspirate, enzymes of the antioxidant system: superoxide dismutase, catalase, glutathione peroxidase, deoxyribonuclease I (DNase), by spectrophotometric method, remodeling factors – matrix metalloproteinases MMP-2, -9 by flow fluorimetry. Results. In patients of group 1, according to transvaginal echography of the pelvic organs, M-echo was 6.29±1.22 mm, in group 2 – 9.10±1.15 mm. In chronic endometritis, disruption of pro-oxidant and activity of antioxidant factors in the endometrium in women with chronic endometritis and a history of reproductive losses. In the heptane fraction of the phenolic extract, the concentration of primary products of lipid peroxidation (diene conjugates) was 12.98% higher than in the control, the content of secondary products of lipid peroxidation (ketodienes) was higher by 15.45%, conjugated trienes by 18.67% higher than the values in the control group, which indicates increased lipid peroxidation of endometrial cell membranes during CE. In the isopropanol phase, the products of lipid peroxidation of phospholipid acyl residues are detected. In the isopropanol fraction of the lipid extract, the amount of diene conjugates was higher, ketodienes by 24.32% and conjugated trienes by 26.14%. An increase in the activity of the enzymes superoxide dismutase by 23.98%, catalase by 14.34%, glutathione peroxidase by 12.39%, and DNase by 2.9 times was recorded compared to the indicators of group 2. Conclusion. Chronic endometritis is accompanied by changes in the system of pro-oxidant and antioxidant factors, the content of tissue remodeling products. The pathogenetic changes underlying oxidative stress in the endometrium may be increased leukocyte infiltration, imbalance of primary, secondary and end products of lipid peroxidation, enzymes superoxide dismutase, catalase, glutathione peroxidase, deoxyribonuclease I (DNase), expression of matrix metalloproteinases-2, -9.
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来源期刊
Gynecology
Gynecology Medicine-Obstetrics and Gynecology
CiteScore
0.70
自引率
0.00%
发文量
52
审稿时长
8 weeks
期刊最新文献
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