体生长抑素基因免疫改变下丘脑-垂体-肝脏基因表达并促进大足黑山羊生长

IF 2.4 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Animal Bioscience Pub Date : 2024-11-01 Epub Date: 2024-06-26 DOI:10.5713/ab.24.0121
Ge Qin, Shiyong Fang, Xianqing Song, Li Zhang, Jiazhuo Huang, Yongfu Huang, Yanguo Han
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引用次数: 0

摘要

目的:体生长抑素(SS)通过影响生长激素(GH)的合成和分泌,对动物的生长和繁殖起着重要的调节作用。然而,SS调节山羊生长发育的机制仍不清楚:在本研究中,我们随机挑选了 8 只体重相近的 7 月龄大足黑山羊(DBGs),平均分配 4 只公羊作为免疫组和阴性对照组。免疫组口服伤寒沙门氏菌减毒疫苗CSO22(ptCS/2SS-asd),阴性对照组口服空载体疫苗CSO22(pVAX-asd):结果:免疫组山羊血清中的 SS 浓度明显低于阴性对照组,日增重明显高于阴性对照组(p < 0.05)。免疫 SS-14 DNA 疫苗后,山羊血清中生长相关激素(如促生长激素和 IGF-1)的浓度明显升高(p < 0.05)。口服 SS-14 DNA 疫苗和阴性对照 DBGs 的下丘脑的 RNA-seq 分析发现了 31 个差异表达基因(DEGs)。脑垂体发现了 164 个 DEGs。肝脏通过 RNA-seq 共检测到 246 个 DEGs。DEGs的基因本体(GO)富集于线粒体包膜、细胞外区域、受体结合和细胞增殖。通过京都基因和基因组百科全书分析,探索了与 DEGs 相关的生物代谢途径。DEGs与代谢途径、氧化磷酸化、维生素消化吸收和半乳糖代谢有关。这些候选基因(如 DGKK、CYTB、DUSP1 和 LRAT)可为探索 SS 促进生长和发育的分子机制提供参考:总之,这些结果表明,SS DNA 疫苗通过改变与生长相关的激素浓度和调节下丘脑-垂体-肝轴中与生长相关的基因的表达,促进了 DBGs 的生长。
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Immunisation of the somatostatin gene alters hypothalamic-pituitary-liver gene expressions and enhances growth in Dazu black goats.

Objective: Somatostatin (SS) plays important regulatory roles in animal growth and reproduction by affecting the synthesis and secretion of growth hormone (GH). However, the mechanism by which SS regulates growth and development in goats is still unclear.

Methods: In this study, we randomly selected eight 7-month-old Dazu black goats (DBGs) of similar body weight and equally assigned four bucks as the immunised and negative control groups. The immunised group received the Salmonella typhi attenuated vaccine X9241 (ptCS/2SS-asd) orally, whilst the negative control group received the empty vector vaccine X9241 (pVAX-asd) orally.

Results: The SS concentration in the serum of goats in the immunised group was significantly lower than that in the negative control group, and the daily gain was significantly higher (p<0.05). SS-14 DNA vaccine immunisation resulted in significantly higher concentrations of growth-related hormones such as GH-releasing hormone and insulin growth factor 1 (IGF-1) in the serum of goats (p<0.05). RNA-seq analysis of hypothalamus of oral SS-14 DNA vaccine and negative control DBGs identified 31 differentially expressed genes (DEGs). Pituitary gland identified 164 DEGs. A total of 246 DEGs were detected in the liver by RNA-seq. Gene ontology of DEGs was enriched in mitochondrial envelope, extracellular region, receptor binding and cell proliferation. The biological metabolic pathways associated with DEGs were explored by Kyoto encyclopedia of genes and genomes analysis. DEGs were associated with metabolic pathways, oxidative phosphorylation, vitamin digestion and absorption and galactose metabolism. These candidate genes (e.g. DGKK, CYTB, DUSP1, and LRAT) may provide references for exploring the molecular mechanisms by which SS promotes growth and development.

Conclusion: Overall, these results demonstrated that the SS DNA vaccine enhanced the growth of DBGs by altering growth-related hormone concentrations and regulating the expression of growth-related genes in the hypothalamic-pituitary-liver axis.

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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
5.00
自引率
0.00%
发文量
223
审稿时长
3 months
期刊最新文献
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