鉴定人体微生物群中的 3-Ketocapnine 还原酶活性。

IF 4.5 Q1 MICROBIOLOGY mLife Pub Date : 2024-06-28 eCollection Date: 2024-06-01 DOI:10.1002/mlf2.12134
Xiaotong Wu, Lukuan Hou, Haili Zhang, Yi Ma, Jufang Wang, Mingwei Cai, Xiaoyu Tang
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引用次数: 0

摘要

微生物在人体内合成的磺脂类很可能与维持人体健康或导致疾病有关。然而,负责其生物合成的酶在很大程度上仍不为人所知。在这项研究中,我们发现并验证了 3-酮巯基还原酶(第三步酶)在体内和体外将 l-磷酸丝氨酸转化为磺胺酸 B 的四步转化过程中的作用。这一发现建立在我们以前对磺脂类生物合成的研究基础之上,以前的研究主要集中在阴道细菌 Chryseobacterium gleum DSM 16776 和肠道细菌 Alistipes finegoldii DSM 17242 上。通过全面的基因图谱绘制,我们证明了潜在的磺脂类生物合成基因广泛存在于栖息在人体不同区域的不同细菌物种中。这些发现揭示了人体微生物群中磺脂类代谢物的普遍性,表明这些脂质分子在影响人体复杂的微生物生态系统中错综复杂的生物相互作用方面具有潜在的作用。
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Identification of 3-ketocapnine reductase activity within the human microbiota.

The microbial synthesis of sulfonolipids within the human body is likely involved in maintaining human health or causing diseases. However, the enzymes responsible for their biosynthesis remain largely unknown. In this study, we identified and verified the role of 3-ketocapnine reductase, the third-step enzyme, in the four-step conversion of l-phosphoserine into sulfobacin B both in vivo and in vitro. This finding builds upon our previous research into sulfonolipid biosynthesis, which focused on the vaginal bacterium Chryseobacterium gleum DSM 16776 and the gut bacterium Alistipes finegoldii DSM 17242. Through comprehensive gene mapping, we demonstrate the widespread presence of potential sulfonolipid biosynthetic genes across diverse bacterial species inhabiting various regions of the human body. These findings shed light on the prevalence of sulfonolipid-like metabolites within the human microbiota, suggesting a potential role for these lipid molecules in influencing the intricate biointeractions within the complex microbial ecosystem of the human body.

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