过氧乙酸在细菌缓冲溶液中和甜椒上抗李斯特菌的功效

IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of Agriculture and Food Research Pub Date : 2024-06-24 DOI:10.1016/j.jafr.2024.101265
Peighton Foster, Corey Coe, Carly Long, Md Shafiul Islam Rion, Gary Freshour, Annette Freshour, Jacek Jaczynski, Kristen Matak, Cangliang Shen
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引用次数: 0

摘要

本研究旨在:1)评估过氧乙酸(PAA)灭活 0.1 % 缓冲蛋白胨水中的李斯特菌的效果;2)评估减少和减轻辣椒交叉污染的效果。在研究-1 中,将 1.0 毫升 PAA 溶液(133、265 和 448 ppm)的等分试样加入 8 带深孔微孔板的前 6 个孔中。然后,加入 0.1 毫升经序列稀释的耐萘啶酸性(NaL)单核细胞增多性乳酸杆菌,并立即用多通道移液管混合。暴露 0、5、15、30 至 120 秒后,加入 1 毫升 2 × D/E 中和溶液终止反应,然后平铺到胰蛋白酶大豆琼脂 +200 ppm NaL 上,在 35 °C 下培养 48 小时。在分别暴露于 133、265 和 448 ppm PAA 120、90 和 60 秒后,单核细胞增多症菌落总数分别为 0.3 log10 CFU/ml,并符合线性和威布尔模型。与仅用水处理相比,使用 23-96 ppm PAA 可使单核细胞增多症菌落总数减少 2.03-3.32 log10MPN/g,交叉污染细胞数减少 2.36-3.17 log10MPN/g,且无差异(P > 0.05)。结果表明,在三重清洗过程中使用允许浓度(23-96 ppm)的 PAA 对辣椒的抗李斯特菌活性并不乐观。未来的研究需要确定 PAA 溶液的最低浓度,以有效减少和减轻辣椒上李斯特菌的微生物交叉感染。
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Anti-Listeria efficacy of peroxyacetic acid in bacterial buffered solution and on bell peppers

This study aims to 1) evaluate the efficacy of peroxyacetic acid (PAA) to inactivate Listeria monocytogenes in 0.1 % buffered-peptone-water; and 2) evaluate the reduction and mitigation of cross-contamination on peppers. In study-1, aliquots of 1.0-ml PAA solutions (133, 265, and 448 ppm) were added to the first 6 wells of 8-strip-deep well microplates. Then, 0.1 ml of serially diluted nalidixic-acid-resistant (NaL) L. monocytogenes was added and mixed immediately with a multichannel pipette. After exposure for 0, 5, 15, 30, and up to 120-s, 1 ml of 2 × D/E neutralized solution was added to terminate the reaction followed by spread-plating onto tryptic soy agar +200 ppm NaL and incubated at 35 °C for 48 h. In study-2, 5 inoculated red peppers were triple-washed with 15 uninoculated green peppers with 0, 23, 53, and 96 ppm of PAA and analyzed using MPN-method. L. monocytogenes counts were <0.3 log10 CFU/ml after exposure to 133, 265, and 448 ppm of PAA for 120, 90 and 60 s, respectively and fit the Linear and Weibull Models. Applying 23–96 ppm of PAA reduced L. monocytogenes by 2.03–3.32 log10MPN/g and transferred cross-contaminated cell counts by 2.36–3.17 log10MPN/g, with no differences (P > 0.05) compared to the water only treatment. Results suggested that applying allowable concentrations (23–96 ppm) of PAA during triple-wash does not show promising anti-Listeria activity on peppers. Future studies are needed to determine the minimum concentrations of the PAA solution required to effectively reduce and mitigate microbial cross-contamination of Listeria monocytogenes on peppers.

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来源期刊
CiteScore
5.40
自引率
2.60%
发文量
193
审稿时长
69 days
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