利用图像处理技术定量测量等离子处理下 ROS 对模型组织的渗透情况

IF 2.6 3区 物理与天体物理 Q3 ENGINEERING, CHEMICAL Plasma Chemistry and Plasma Processing Pub Date : 2024-06-25 DOI:10.1007/s11090-024-10488-2
Bingkai Wang, Nan Zhang, Chengfeng Xiong, Xu Yan, Zilan Xiong
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引用次数: 0

摘要

血浆剂量定量是血浆医学临床的核心问题之一。在临床上,等离子体中的活性物种进入被处理物体的时空分布和总剂量尤为重要。在这项研究中,我们开发了一种基于图像处理技术的测量方案,用于量化活性氧(ROS)对模型组织的穿透剂量,并分析了治疗条件对浓度分布和总量的影响。首先,通过图像处理和滴定实验建立颜色指数与 ROS 浓度之间的数值关系,计算出 ROS 在被处理样品各切片层上的空间浓度分布。然后,通过对纵向切片组织图像进行图像分割,得出 ROS 的渗透深度。最后,通过整合各层的浓度和深度,得出 ROS 的绝对量。在氩等离子体喷射治疗中,穿透深度和绝对量与治疗时间呈正相关,与治疗距离呈负相关。在设定条件下,穿透深度范围为 0.5-3 mm,总剂量为 0.05-0.47 µmol。通过与溶解在液体中的紫外可见光法测得的 ROS 总量进行比较,证实了所提方法的有效性,为等离子体剂量定量问题提供了新的解决方案,同时也有利于了解等离子体与组织之间的相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Quantitative Measurement of ROS Penetration into Model Tissue Under Plasma Treatment Using Image Processing

Plasma dose quantification is one of the core problems in clinical of plasma medicine. The spatial-temporal distribution and the total dose of the reactive species from plasma into the processed object are especially important in clinic. In this study, we developed a measurement scheme based on image processing technology for quantifying the penetration dose of reactive oxygen species (ROS) into model tissues, and analyzed the effects of treatment conditions on the concentration distribution and the total amount. First, by establishing a numerical relationship between the color index and ROS concentration through image processing and titration experiment, the spatial concentration distribution of ROS on each sliced layer of the treated sample was calculated. Then, the ROS penetration depth was obtained through image segmentation of longitudinal sliced tissue image. Finally, by integrating the concentration of each layer and the depth, the absolute amount of ROS was obtained. Both the penetration depth and absolute amount exhibit a positive correlation with treatment time and a negative correlation with treatment distance under an Ar plasma jet treatment. A range of penetration depth of 0.5–3 mm and total dose of 0.05–0.47 µmol was obtained under the setting conditions. The effectiveness of the proposed method was confirmed by comparing with the total ROS amount measured by UV-Vis method dissolved in liquid, providing a new solution for the issue in plasma dose quantification, and is also benefit for the understanding of plasma-tissue interaction.

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来源期刊
Plasma Chemistry and Plasma Processing
Plasma Chemistry and Plasma Processing 工程技术-工程:化工
CiteScore
5.90
自引率
8.30%
发文量
73
审稿时长
6-12 weeks
期刊介绍: Publishing original papers on fundamental and applied research in plasma chemistry and plasma processing, the scope of this journal includes processing plasmas ranging from non-thermal plasmas to thermal plasmas, and fundamental plasma studies as well as studies of specific plasma applications. Such applications include but are not limited to plasma catalysis, environmental processing including treatment of liquids and gases, biological applications of plasmas including plasma medicine and agriculture, surface modification and deposition, powder and nanostructure synthesis, energy applications including plasma combustion and reforming, resource recovery, coupling of plasmas and electrochemistry, and plasma etching. Studies of chemical kinetics in plasmas, and the interactions of plasmas with surfaces are also solicited. It is essential that submissions include substantial consideration of the role of the plasma, for example, the relevant plasma chemistry, plasma physics or plasma–surface interactions; manuscripts that consider solely the properties of materials or substances processed using a plasma are not within the journal’s scope.
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