Biosynthesized silver nanoparticles using Jojoba oil as a novel antibacterial agent for controlling Pectobacterium carotovorum in potato

The current work describes a biological method to create silver nanoparticles (AgNPs) using jojoba oil. As a reducing and capping agent, jojoba oil was utilized in the production of AgNPs. AgNPs may be classified by their UV-VIS, FTIR, and spectra. A transmission electron microscope (TEM) was used to examine the shape and size of the silver nanoparticles. The average size of the synthesized silver nanoparticles was 25.6 ± 10.3 nm, and they had a spherical shape. According to FTIR spectroscopy tests, the silver nanoparticles' surface had organic molecules bound to it. Five bacterial isolates were isolated and tested for pathogenicity on potatoes. All isolates produced the typical symptoms of soft rot disease. Isolate Pcc2 caused the highest disease severity (45%). According to 16sRNA, isolate Pcc2 was identified as Pectobacterium carotovorum subsp. carotovorum. Increasing the dosage of AgNPs increased the antibacterial effect against P. carotovorum. From these results, we can conclude that the use of AgNPs is a safe method for controlling postharvest potato diseases caused by Pectobacterium carotovorum subsp. carotovorum.