从牙髓干细胞中提取的新亚型人工细胞衍生囊泡与细胞外囊泡相比,具有生物等效性和更高的获取效率。

IF 15.5 1区 医学 Q1 CELL BIOLOGY Journal of Extracellular Vesicles Pub Date : 2024-07-04 DOI:10.1002/jev2.12473
Xingxiang Duan, Rui Zhang, Huixian Feng, Heng Zhou, Yu Luo, Wei Xiong, Junyi Li, Yan He, Qingsong Ye
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引用次数: 0

摘要

从牙髓干细胞(DPSC)中提取的细胞外囊泡(EVs)已在多种疾病模型中显示出卓越的疗效。然而,目前的生产方法无法满足临床治疗的需要。在本研究中,我们提出了一种创新方法,通过提取和纯化牙髓干细胞裂解物释放的内容物(即细胞内囊泡),大幅提高 "人工细胞衍生囊泡(ACDVs)"的产量。对ACDVs和超速离心法获得的ACDVs进行了比较分析。从细胞裂解液中提取的ACDV符合EV的一般标准,并具有相似的蛋白质分泌特征。新的 ACDV 与 EVs 一样,也能显著促进伤口愈合、增加或减少胶原蛋白再生,并减少炎症因子的产生。更重要的是,与使用超速离心法提取的 EVs 相比,提取效率提高了 16 倍。由于其令人印象深刻的特性,这种新亚型 ACDVs 成为再生医学未来临床应用的潜在候选者。
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A new subtype of artificial cell-derived vesicles from dental pulp stem cells with the bioequivalence and higher acquisition efficiency compared to extracellular vesicles

Extracellular vesicles (EVs) derived from dental pulp stem cells (DPSC) have been shown an excellent efficacy in a variety of disease models. However, current production methods fail to meet the needs of clinical treatment. In this study, we present an innovative approach to substantially enhance the production of ‘Artificial Cell-Derived Vesicles (ACDVs)’ by extracting and purifying the contents released by the DPSC lysate, namely intracellular vesicles. Comparative analysis was performed between ACDVs and those obtained through ultracentrifugation. The ACDVs extracted from the cell lysate meet the general standard of EVs and have similar protein secretion profile. The new ACDVs also significantly promoted wound healing, increased or decreased collagen regeneration, and reduced the production of inflammatory factors as the EVs. More importantly, the extraction efficiency is improved by 16 times compared with the EVs extracted using ultracentrifuge method. With its impressive attributes, this new subtype of ACDVs emerge as a prospective candidate for the future clinical applications in regenerative medicine.

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来源期刊
Journal of Extracellular Vesicles
Journal of Extracellular Vesicles Biochemistry, Genetics and Molecular Biology-Cell Biology
CiteScore
27.30
自引率
4.40%
发文量
115
审稿时长
12 weeks
期刊介绍: The Journal of Extracellular Vesicles is an open access research publication that focuses on extracellular vesicles, including microvesicles, exosomes, ectosomes, and apoptotic bodies. It serves as the official journal of the International Society for Extracellular Vesicles and aims to facilitate the exchange of data, ideas, and information pertaining to the chemistry, biology, and applications of extracellular vesicles. The journal covers various aspects such as the cellular and molecular mechanisms of extracellular vesicles biogenesis, technological advancements in their isolation, quantification, and characterization, the role and function of extracellular vesicles in biology, stem cell-derived extracellular vesicles and their biology, as well as the application of extracellular vesicles for pharmacological, immunological, or genetic therapies. The Journal of Extracellular Vesicles is widely recognized and indexed by numerous services, including Biological Abstracts, BIOSIS Previews, Chemical Abstracts Service (CAS), Current Contents/Life Sciences, Directory of Open Access Journals (DOAJ), Journal Citation Reports/Science Edition, Google Scholar, ProQuest Natural Science Collection, ProQuest SciTech Collection, SciTech Premium Collection, PubMed Central/PubMed, Science Citation Index Expanded, ScienceOpen, and Scopus.
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