牛至精油及其主要成分香芹酚对 HepG2 细胞毒性通路基因表达的影响

2区 医学 Q1 Medicine BMC Complementary and Alternative Medicine Pub Date : 2024-07-11 DOI:10.1186/s12906-024-04571-6
Özlem Tomsuk, Victor Kuete, Hülya Sivas, Mine Kürkçüoğlu
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引用次数: 0

摘要

牛至品种在各种商业建筑中被用作治疗烧伤和创伤、农业、酒精饮料、香料和食品调味物质。牛至精油(EOOO)及其成分香芹酚(CV)具有广泛的生物活性,包括抗癌活性。本研究的目的是调查牛至精油及其主要成分 CV 的生长抑制活性,以及 HepG2 细胞中的肝毒性通路相关基因。研究采用胰蓝排除/溴脱氧尿苷(BrdU)掺入试验和实时聚合酶链反应(RT-PCR)阵列,分别检测了 EOOO 和 CV 对 HepG2 细胞生长和 84 个肝毒性通路相关基因 mRNA 表达的影响。实时反转录聚合酶链反应(RT2-PCR)阵列分析表明,84 个基因中有 32 个基因的表达在 EOOO 处理的细胞中发生了至少 2 倍或以上的变化。其中,17 个基因的表达水平升高,15 个基因的表达水平降低。此外,细胞暴露于 45 微克/毫升的 CV 后,8 个基因的表达水平升高,而另外 8 个基因的表达水平降低。香叶精油和香芹酚都影响了 HepG2 细胞中参与肝毒性途径的 48 个基因的表达,这表明它们具有保肝作用,并可能具有抗肝癌作用。本研究表明,牛至精油和香芹酚具有非肝脏毒性,可用于抗癌或草药等多种用途。
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Effects of essential oil of Origanum onites and its major component carvacrol on the expression of toxicity pathway genes in HepG2 cells
Origanum species have been used in various commercial constructions as a remedy against burns and wounds, agriculture, alcoholic drinks, fragrance, and flavoring substances of food products. The essential oil of Origanum onites L. (EOOO) and its component carvacrol (CV) possesses a wide range of biological activities including anti-cancer activity. The purpose of this study was to investigate the growth inhibitory activity of the essential oil and its major component CV and then hepatotoxicity pathway-related genes in HepG2 cells. The effects of the EOOO and CV on cell growth and mRNA expressions of 84 hepatotoxicity pathway-related genes were investigated in HepG2, using trypan blue exclusion/ bromodeoxyuridine (BrdU) incorporation tests and real-time-polymerase chain reaction (RT-PCR) array, respectively. The EOOO and CV inhibited cell growth with IC50 values of 0.08 µg/mL and 45 µg/mL, respectively, after 24 h. Real-time, reverse-transcription-polymerase chain reaction (RT2-PCR) array analysis revealed that expressions of 32 genes out of 84 were changed at least 2-fold or more in the EOOO-treated cells. Among them, expression levels of 17 genes were elevated, while expression levels of 15 genes were diminished. Furthermore, after exposure of cells to 45 µg/mL of CV, the expression of 8 genes was increased while the other 8 genes were decreased. Both the EOOO and carvacrol affected the expression of 48 genes of HepG2 cells which are involved in the hepatotoxicity pathway, indicating their hepatoprotective and possible anti-hepatocarcinogenic effects. The present study demonstrates that the essential oil of Origanum onites and carvacrol can be used in various applications such as anticancer or herbal drugs, since its non-hepatotoxicity.
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来源期刊
BMC Complementary and Alternative Medicine
BMC Complementary and Alternative Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
7.00
自引率
0.00%
发文量
0
审稿时长
3 months
期刊介绍: BMC Complementary Medicine and Therapies is an open access journal publishing original peer-reviewed research articles on interventions and resources that complement or replace conventional therapies, with a specific emphasis on research that explores the biological mechanisms of action, as well as their efficacy, safety, costs, patterns of use and/or implementation.
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