树蛙的 DNA 条形码：测试 Hyla savignyi 是否存在两个线粒体系谱

IF 0.5 4区生物学 Q4 BIOLOGY Biology Bulletin Pub Date : 2024-07-13 DOI:10.1134/s1062359024606980

T. Ergül Kalaycı

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引用次数: 0

摘要

摘要DNA 条形码是一种流行的方法，可帮助鉴定物种，并已发现了许多两栖动物新物种。在这项研究中，我们首次对土耳其安纳托利亚地区的两个水龙科代表物种（Hyla orientalis 和 Hyla savignyi）进行了 DNA 条形编码。我们应用了五种物种划分测试（ABGD、GMYC、PTP、RESL 和统计解析分析），其中四种测试结果表明安纳托利亚地区存在 Hylidae 的三个线粒体系。种内遗传距离（K2P）在 0.0014 至 0.0045 之间，种间遗传距离在 0.0753 至 0.1933 之间。根据最大似然法（ML）得到的树拓扑结构，在安纳托利亚的 H. savignyi 中可以区分出两个品系，这些品系是否代表不同的物种还需要进一步研究。

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DNA Barcoding of Tree Frogs: Testing the Existence of Two Mitochondrial Lineages of Hyla savignyi

Abstract

DNA barcoding is a popular approach that can aid in identifying species and has led to the discovery of many new amphibian species. In this study, we first performed DNA barcoding for two Hylidae family representatives (Hyla orientalis and Hyla savignyi) in the Anatolia region of Türkiye. Five species delineation tests (ABGD, GMYC, PTP, RESL, and statistical parsimony analysis) were applied, and four of them indicated that three mitochondrial lineages of Hylidae are present in Anatolia. Intraspecific genetic distances (K2P) ranged from 0.0014 to 0.0045, while the interspecific genetic distances ranged from 0.0753 to 0.1933. According to the tree topologies obtained from maximum likelihood (ML) within H. savignyi from Anatolia, two lineages can be differentiated, and further investigation is warranted as to whether these lineages represent distinct species.

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来源期刊

Biology Bulletin 生物-生物学

CiteScore

0.70

自引率

20.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Biology Bulletin (Izvestiya Rossiiskoi Akademii Nauk – Seriya Biologicheskaya) is an interdisciplinary journal of general biology. It focuses on fundamental studies in the fields of cell biology, biochemistry, zoology, botany, physiology, and ecology. This journal publishes current materials of experimental studies and surveys on current problems in general biology. It also publishes information on scientific conferences and new books in the fields of general biology.