对南美白对虾(Metapenaeus stebbingi)弧菌病的认识:流行率、致病基因和在肉中存在的潜在局限性

S. M. Mansour, Rehab E. M. Gaafar, Walaa A. El - Shaer
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摘要

弧菌是包括虾在内的所有甲壳类动物中分布最广的物种,虾对弧菌易感。许多弧菌与食源性疾病有关,对人类有害。本研究旨在确定从南美白对虾(Metapenaeus stebbingi)中分离出的弧菌的流行率和毒力基因,并测试一些有机酸对其存活的影响,以延长对虾的保质期。2023 年 7 月至 11 月期间,从埃及伊斯梅利亚市的渔民处采集了南美白对虾(M. stebbingi)样本。使用特定培养基(硫代硫酸钠-柠檬酸盐-胆汁-蔗糖琼脂)和生化测试,从虾的肝胰腺和肌肉组织中分离和鉴定弧菌种类。然后,通过聚合酶链式反应确认其身份并检测其毒力基因。此外,还进行了醋酸和柠檬酸处理试验,以降低人工接种虾在不同处理持续时间内的副溶血性弧菌数量,并延长冷冻虾在冷藏储存(4 o C)条件下的货架期。在间隔 0、3、6、9 和 12 天的冷藏条件下,对感官属性、pH 值、需氧菌落总数和脂质氧化进行了评估。结果显示,在自然感染的南美白对虾(M. stebbingi)中发现了副溶血性弧菌和藻溶弧菌,总感染率为 14%,其中藻溶弧菌感染率最高,占 9.6 %。
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Insights on Vibriosis in white shrimp (Metapenaeus stebbingi): prevalence, virulence genes, and potential limitations of Existence in the meat
V ibrios are the most widespread species of all crustaceans, including shrimp, which are susceptible. Numerous Vibrio species are linked to food - borne illnesses and are harmful to humans. The present investigation was performed to identify the prevalence and virulence genes of Vibrio species isolated from white shrimp (Metapenaeus stebbingi) and test the efficacy of some organic acids on its survival with improving shrimp shelf life. Samples of white shrimp (M. stebbingi) were collected from fisher-men in Ismailia City, Egypt, between July and November 2023. Vibrio species were isolated and identified from the shrimp's hepatopancreas and musculature using its specific media (Thiosulfate - Citrate - Bile - Sucrose Agar) and biochemical tests. Then, the identification was confirmed and their virulence genes were detected by using PCR. Moreover, trials were performed by using acetic and citric acids treatments for decreasing Vibrio parahaemolyticus counts in artificially inoculated shrimp at different treatment durations, as well as for extending shelf life of chilled shrimp at refrigeration storage (4 o C). The sensory attributes, pH values, total aerobic plate counts and lipid oxidation were evaluated under refrigeration at interval 0, 3, 6, 9 and 12 days. Results revealed that Vibrio parahaemolyticus and Vibrio alginolyticus were found to be present in naturally infected white shrimp (M. stebbingi), with a total prevalence of 14%, where V. alginolyticus was the most prevalent, accounting for 9.6 ,%
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