对环境分离的弧菌的致病性和毒力进行体内检测。

IF 3.9 3区 生物学 Q2 MICROBIOLOGY MicrobiologyOpen Pub Date : 2024-07-23 DOI:10.1002/mbo3.1427
Shannon E. Pipes, Charles R. Lovell, Katie L. Kathrein
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引用次数: 0

摘要

弧菌是一种嗜卤的革兰氏阴性环境病原体,人类接触弧菌的机会越来越多。尽管如此,其致病性和毒性机制在很大程度上仍不为人所知。每年都会发生数百起与弧菌有关的感染,92%的病例会导致住院治疗,死亡率高达 35%。这种感染很严重,通常是通过食用受污染的食物或开放性伤口接触受污染的水而感染。这可能导致坏死性筋膜炎,需要截肢受感染的组织。虽然有几个基因(rtxA1、vvpE 和 vvhA)与这种生物的致病性有关,但尚未发现明确的机制。在本研究中,我们利用斑马鱼模型(Danio rerio)检测了环境分离的弧菌菌株,以研究它们的致病能力。我们发现,不同菌株之间的毒力差异很大。常用的致病菌株标记基因 vcgC 无法准确预测毒性更强的菌株。值得注意的是,本研究中毒力最低的菌株--弧菌 Sept WR1-BW6,其 vcgC、vvhA 和 rtxA1 检测结果均呈阳性,但并未导致鱼类严重发病,也是唯一没有导致鱼类死亡的菌株。我们的研究表明,不同环境中的菌株毒力差异很大,不能仅根据基因型来准确预测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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In vivo examination of pathogenicity and virulence in environmentally isolated Vibrio vulnificus

Human exposure to Vibrio vulnificus, a gram-negative, halophilic environmental pathogen, is increasing. Despite this, the mechanisms of its pathogenicity and virulence remain largely unknown. Each year, hundreds of infections related to V. vulnificus occur, leading to hospitalization in 92% of cases and a mortality rate of 35%. The infection is severe, typically contracted through the consumption of contaminated food or exposure of an open wound to contaminated water. This can result in necrotizing fasciitis and the need for amputation of the infected tissue. Although several genes (rtxA1, vvpE, and vvhA) have been implicated in the pathogenicity of this organism, a defined mechanism has not been discovered. In this study, we examine environmentally isolated V. vulnificus strains using a zebrafish model (Danio rerio) to investigate their virulence capabilities. We found significant variation in virulence between individual strains. The commonly used marker gene of disease-causing strains, vcgC, did not accurately predict the more virulent strains. Notably, the least virulent strain in the study, V. vulnificus Sept WR1-BW6, which tested positive for vcgC, vvhA, and rtxA1, did not cause severe disease in the fish and was the only strain that did not result in any mortality. Our study demonstrates that virulence varies greatly among different environmental strains and cannot be accurately predicted based solely on genotype.

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来源期刊
MicrobiologyOpen
MicrobiologyOpen MICROBIOLOGY-
CiteScore
8.00
自引率
0.00%
发文量
78
审稿时长
20 weeks
期刊介绍: MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era. The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes. MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to: - agriculture - antimicrobial resistance - astrobiology - biochemistry - biotechnology - cell and molecular biology - clinical microbiology - computational, systems, and synthetic microbiology - environmental science - evolutionary biology, ecology, and systematics - food science and technology - genetics and genomics - geobiology and earth science - host-microbe interactions - infectious diseases - natural products discovery - pharmaceutical and medicinal chemistry - physiology - plant pathology - veterinary microbiology We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses. The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations. MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.
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