{"title":"影响口腔的组织细胞肉瘤:临床、病理和分子研究。","authors":"","doi":"10.1016/j.oooo.2024.06.014","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p><span>To investigate the clinicopathological, immunohistochemical and molecular features of histiocytic sarcomas affecting the </span>oral cavity.</p></div><div><h3>Methods</h3><p><span>Pathology files of two institutions were searched for cases of histiocytic sarcoma, and new H&E-stained slides and immunohistochemistry reactions evaluated for diagnosis confirmation. Molecular screening for </span><em>KRAS</em> and <em>PIK3CA</em><span><span> mutations was performed through polymerase chain reaction (PCR) followed by </span>Sanger sequencing. </span><em>BRAFp.V600E</em><span> mutation was assessed by pyrosequencing. Clinical data regarding sex, age, tumor location, systemic manifestations, clinical presentation, follow-up time, treatment applied and status at last follow-up were collected from patients' pathology and medical files.</span></p></div><div><h3>Results</h3><p><span><span>Three cases were retrieved during the period investigated (2000-2023). Two females and one male were affected, with a wide age range, involving the tongue<span>, palate and </span></span>gingiva<span>. Histopathologically, the neoplasms presented as highly pleomorphic atypical cells distributed diffusely with infiltration of normal structures. All cases demonstrated histiocytic differentiation expressing CD68<span> and CD163, and a high Ki67 expression. Genetic mutations were evaluated in two cases. One case harboured </span></span></span><em>BRAF-V600E</em> mutation, but not in <em>KRAS</em> and <em>PIK3CA</em>, while the second case did not show mutation in <em>BRAF-V600E, KRAS</em> and <em>PI3KCA</em>. One patient was lost, and two patients died after eight and four months of follow-up.</p></div><div><h3>Conclusion</h3><p>Histiocytic sarcomas involving the oral cavity are extremely rare, and may represent dissemination of a systemic condition. It has an aggressive biological behaviour with a poor overall prognosis.</p></div>","PeriodicalId":49010,"journal":{"name":"Oral Surgery Oral Medicine Oral Pathology Oral Radiology","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Histiocytic sarcoma affecting the oral cavity: a clinical, pathologic and molecular study\",\"authors\":\"\",\"doi\":\"10.1016/j.oooo.2024.06.014\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p><span>To investigate the clinicopathological, immunohistochemical and molecular features of histiocytic sarcomas affecting the </span>oral cavity.</p></div><div><h3>Methods</h3><p><span>Pathology files of two institutions were searched for cases of histiocytic sarcoma, and new H&E-stained slides and immunohistochemistry reactions evaluated for diagnosis confirmation. Molecular screening for </span><em>KRAS</em> and <em>PIK3CA</em><span><span> mutations was performed through polymerase chain reaction (PCR) followed by </span>Sanger sequencing. </span><em>BRAFp.V600E</em><span> mutation was assessed by pyrosequencing. Clinical data regarding sex, age, tumor location, systemic manifestations, clinical presentation, follow-up time, treatment applied and status at last follow-up were collected from patients' pathology and medical files.</span></p></div><div><h3>Results</h3><p><span><span>Three cases were retrieved during the period investigated (2000-2023). Two females and one male were affected, with a wide age range, involving the tongue<span>, palate and </span></span>gingiva<span>. Histopathologically, the neoplasms presented as highly pleomorphic atypical cells distributed diffusely with infiltration of normal structures. All cases demonstrated histiocytic differentiation expressing CD68<span> and CD163, and a high Ki67 expression. Genetic mutations were evaluated in two cases. One case harboured </span></span></span><em>BRAF-V600E</em> mutation, but not in <em>KRAS</em> and <em>PIK3CA</em>, while the second case did not show mutation in <em>BRAF-V600E, KRAS</em> and <em>PI3KCA</em>. One patient was lost, and two patients died after eight and four months of follow-up.</p></div><div><h3>Conclusion</h3><p>Histiocytic sarcomas involving the oral cavity are extremely rare, and may represent dissemination of a systemic condition. It has an aggressive biological behaviour with a poor overall prognosis.</p></div>\",\"PeriodicalId\":49010,\"journal\":{\"name\":\"Oral Surgery Oral Medicine Oral Pathology Oral Radiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-06-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Oral Surgery Oral Medicine Oral Pathology Oral Radiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212440324003687\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Oral Surgery Oral Medicine Oral Pathology Oral Radiology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212440324003687","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Histiocytic sarcoma affecting the oral cavity: a clinical, pathologic and molecular study
Objective
To investigate the clinicopathological, immunohistochemical and molecular features of histiocytic sarcomas affecting the oral cavity.
Methods
Pathology files of two institutions were searched for cases of histiocytic sarcoma, and new H&E-stained slides and immunohistochemistry reactions evaluated for diagnosis confirmation. Molecular screening for KRAS and PIK3CA mutations was performed through polymerase chain reaction (PCR) followed by Sanger sequencing. BRAFp.V600E mutation was assessed by pyrosequencing. Clinical data regarding sex, age, tumor location, systemic manifestations, clinical presentation, follow-up time, treatment applied and status at last follow-up were collected from patients' pathology and medical files.
Results
Three cases were retrieved during the period investigated (2000-2023). Two females and one male were affected, with a wide age range, involving the tongue, palate and gingiva. Histopathologically, the neoplasms presented as highly pleomorphic atypical cells distributed diffusely with infiltration of normal structures. All cases demonstrated histiocytic differentiation expressing CD68 and CD163, and a high Ki67 expression. Genetic mutations were evaluated in two cases. One case harboured BRAF-V600E mutation, but not in KRAS and PIK3CA, while the second case did not show mutation in BRAF-V600E, KRAS and PI3KCA. One patient was lost, and two patients died after eight and four months of follow-up.
Conclusion
Histiocytic sarcomas involving the oral cavity are extremely rare, and may represent dissemination of a systemic condition. It has an aggressive biological behaviour with a poor overall prognosis.
期刊介绍:
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology is required reading for anyone in the fields of oral surgery, oral medicine, oral pathology, oral radiology or advanced general practice dentistry. It is the only major dental journal that provides a practical and complete overview of the medical and surgical techniques of dental practice in four areas. Topics covered include such current issues as dental implants, treatment of HIV-infected patients, and evaluation and treatment of TMJ disorders. The official publication for nine societies, the Journal is recommended for initial purchase in the Brandon Hill study, Selected List of Books and Journals for the Small Medical Library.