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引用次数: 0
摘要
本研究旨在优化菌株，并评估在体外条件下用不同浓度的激素添加生长培养基对促进狮鬃菇（Hericium erinaceus）菌丝生长的影响。在五株狮头菇菌株中，He-04 菌株的 GRavr 最大，为 4.78 mm d-1 。对马铃薯葡萄糖琼脂（PDA）、麦芽提取物琼脂（MEA）、锯末提取物琼脂（SDEA）、小麦秸秆提取物琼脂（WSEA）和大米秸秆提取物琼脂（RSEA）等不同培养基进行了评估，这些培养基分别添加了四种浓度（10、20、30 和 40 ppm）的赤霉素、激肽和吲哚乙酸，以促进麦角菌丝生长。结果表明，PDA 是促进麦角菌丝生长的最佳培养基。在添加了吲哚乙酸（10 ppm）的 PDA 中观察到最高的菌丝 GRavr 为 8.47 mm d -1 ，其次是赤霉素和激肽（30 ppm），菌丝 GRavr 分别降至 8.15 和 7.75 mm d -1 。研究发现，25°C 的温度和 7.0 的 pH 值最适合麦角菌菌丝生长。
Optimization of Mycelial Growth Parameters in Lion’s Mane Edible Medicinal Mushroom Hericium erinaceus (Agaricomycetes) by Supplementation of Growth Hormones
The present study was carried out to optimize the strain and evaluate the effect
of amendment of growth media with different hormone concentrations for enhancing
mycelium growth of lion’s mane mushroom (Hericium erinaceus) under in vitro conditions. Among the five strains of H. erinaceus, He-04 strain showed maximum GRavr of 4.78 mm d-1 . Different media viz., potato dextrose agar (PDA), malt extract agar (MEA), sawdust extract agar (SDEA), wheat straw extract agar (WSEA) and rice straw extract agar (RSEA) amended with four concentrations (10, 20, 30 and 40 ppm) of gibberellic acid, kinetin and indole acetic acid were evaluated for promotion of mycelial growth of H. erinaceus. PDA was observed to be the best media promoting the mycelial growth of H. erinaceus. The highest mycelial GRavr 8.47 mm d -1 was observed in PDA amended with indole acetic acid (10 ppm) followed by gibberellic acid and kinetin (30 ppm) decreasing mycelial GRav to 8.15 and 7.75mm d -1 respectively. Temperature of 25°C and pH 7.0 was found to be the best for mycelium growth of H. erinaceus.
期刊介绍：
The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.
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