健康老年男性和女性的餐后乳酸穿梭延迟和减少。

IF 4.2 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM American journal of physiology. Endocrinology and metabolism Pub Date : 2024-10-01 Epub Date: 2024-08-07 DOI:10.1152/ajpendo.00183.2024
Jose A Arevalo, Robert G Leija, Adam D Osmond, Casey C Curl, Justin J Duong, Melvin J Huie, Umesh Masharani, George A Brooks
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引用次数: 0

摘要

乳酸盐是糖酵解的产物,在有氧条件下形成。大量研究表明,乳酸盐通量存在于年轻人和运动人群中,但年龄的影响尚不清楚。我们测试了老年人餐后乳酸穿梭(PLS)会减少的假设。我们使用[3-13C]乳酸盐和[6,6-2H]-葡萄糖示踪剂、OGTT 和动脉采血法测定了 15 名年轻(28.1 ± 1.4 岁)和 13 名老年(70.6 ± 2.4 岁)健康男性和女性的餐后乳酸盐出现率(Ra)、消失率(Rd)和氧化率(Rox)。年轻参与者的空腹血液[乳酸][" 0.5 毫摩尔]在葡萄糖挑战后上升,15 分钟达到峰值,30 分钟降至最低点,60 分钟再次上升,达到峰值[" 1.0 毫摩尔]。年龄较大的参与者乳酸Ra的初始反应延迟并减弱,直到90分钟时才上升0.83毫克-千克-1-分钟-1。在整个试验过程中,年轻参与者的乳酸 Rox 较高,两者相差约 0.5 毫克-公斤-1-分钟-1。所有志愿者的乳酸 Ra 和乳酸浓度的初始峰值都表明,在 OGTT 之后存在肠道 PLS。值得注意的是,在全身而非肠道 PLS 阶段,乳酸 Ra 与葡萄糖 Rd 高度相关(r2 = 0.92)。乳酸盐 Ra 和浓度的第二个峰值与葡萄糖 Rd 的对应关系表明乳酸盐 Ra 依赖于葡萄糖 Rd。虽然研究结果表明年轻和年长的研究队列中都存在肠道和全身 PLS 阶段,但在健康的年长者中,新陈代谢反应延迟并减弱。
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Delayed and diminished postprandial lactate shuttling in healthy older men and women.

Lactate, a product of glycolysis, is formed under aerobic conditions. Extensive work has shown lactate flux in young and exercising humans; however, the effect of age is not known. We tested the hypothesis that postprandial lactate shuttling (PLS) would be diminished in older adults. We used [3-13C]lactate and [6,6-2H]glucose tracers, an oral glucose tolerance test (OGTT), and arterialized blood sampling to determine postprandial lactate rates of appearance (Ra), disappearance (Rd), and oxidation (Rox) in 15 young (28.1 ± 1.4 yr) and 13 older (70.6 ± 2.4 yr) healthy men and women. In young participants, fasting blood [lactate] (≈0.5 mM) rose after the glucose challenge, peaked at 15 min, dipped to a nadir at 30 min, and rose again peaking at 60 min (≈1.0 mM). Initial responses in lactate Ra of older participants were delayed and diminished until 90 min rising by 0.83 mg·kg-1·min-1. Lactate Rox was higher throughout the entire trial in young participants by a difference of ∼0.5 mg·kg-1·min-1. Initial peaks in lactate Ra and concentration in all volunteers demonstrated the presence of an enteric PLS following an OGTT. Notably, in the systemic, but not enteric, PLS phase, lactate Ra correlated highly with glucose Rd (r2 = 0.92). Correspondence of second peaks in lactate Ra and concentration and glucose Rd shows dependence of lactate Ra on glucose Rd. Although results show both enteric and systemic PLS phases in young and older study cohorts, metabolic responses were delayed and diminished in healthy older individuals.NEW & NOTEWORTHY We used isotope tracers, an oral glucose tolerance test, and arterialized blood sampling to determine postprandial lactate flux rates in healthy young and older men and women. Lactate rates of appearance and oxidation and the lactate-pyruvate exchange were delayed and diminished in both enteric and systemic postprandial lactate shuttle phases in older participants.

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来源期刊
CiteScore
9.80
自引率
0.00%
发文量
98
审稿时长
1 months
期刊介绍: The American Journal of Physiology-Endocrinology and Metabolism publishes original, mechanistic studies on the physiology of endocrine and metabolic systems. Physiological, cellular, and molecular studies in whole animals or humans will be considered. Specific themes include, but are not limited to, mechanisms of hormone and growth factor action; hormonal and nutritional regulation of metabolism, inflammation, microbiome and energy balance; integrative organ cross talk; paracrine and autocrine control of endocrine cells; function and activation of hormone receptors; endocrine or metabolic control of channels, transporters, and membrane function; temporal analysis of hormone secretion and metabolism; and mathematical/kinetic modeling of metabolism. Novel molecular, immunological, or biophysical studies of hormone action are also welcome.
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